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Packed bed bioreactor for the isolation and expansion of placental-derived mesenchymal stromal cells

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dc.contributor.authorOsiecki, M.
dc.contributor.authorMichl, T.D.
dc.contributor.authorBabur, B.K.
dc.contributor.authorKabiri, M.
dc.contributor.authorAtkinson, K.
dc.contributor.authorLott, W.B.
dc.contributor.authorGriesser, H.J.
dc.contributor.authorDoran, M.
dc.contributor.editorKerkis, I.
dc.date.issued2015
dc.description.abstractLarge numbers of Mesenchymal stem/stromal cells (MSCs) are required for clinical relevant doses to treat a number of diseases. To economically manufacture these MSCs, an automated bioreactor system will be required. Herein we describe the development of a scalable closed-system, packed bed bioreactor suitable for large-scale MSCs expansion. The packed bed was formed from fused polystyrene pellets that were air plasma treated to endow them with a surface chemistry similar to traditional tissue culture plastic. The packed bed was encased within a gas permeable shell to decouple the medium nutrient supply and gas exchange. This enabled a significant reduction in medium flow rates, thus reducing shear and even facilitating single pass medium exchange. The system was optimised in a small-scale bioreactor format (160 cm(2)) with murine-derived green fluorescent protein-expressing MSCs, and then scaled-up to a 2800 cm(2) format. We demonstrated that placental derived MSCs could be isolated directly within the bioreactor and subsequently expanded. Our results demonstrate that the closed system large-scale packed bed bioreactor is an effective and scalable tool for large-scale isolation and expansion of MSCs.
dc.identifier.citationPLoS ONE, 2015; 10(2):1-18
dc.identifier.doi10.1371/journal.pone.0144941
dc.identifier.issn1932-6203
dc.identifier.issn1932-6203
dc.identifier.urihttps://hdl.handle.net/11541.2/122708
dc.language.isoen
dc.publisherPublic Library of Science
dc.relation.fundingInner Wheel Australia
dc.relation.fundingWound Management Innovation CRC 1.06
dc.rightsCopyright 2015 Osiecki et al. This is an openaccess article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. (https://creativecommons.org/licenses/by/4.0/)
dc.source.urihttp://dx.doi.org/10.1371/journal.pone.0144941
dc.subjecthuman bone-marrow
dc.subjectstem-cells
dc.subjectshear-stress
dc.subjectperfusion bioreactor
dc.subjectoxygen-transport
dc.subjectclinical-trials
dc.subjectin-vitro
dc.subjectculture
dc.subjectsystem
dc.subjectdifferentiation
dc.titlePacked bed bioreactor for the isolation and expansion of placental-derived mesenchymal stromal cells
dc.typeJournal article
pubs.publication-statusPublished
ror.fileinfo12143276580001831 13143273130001831 9916101807001831_53139738570001831.pdf
ror.mmsid9916101807001831

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