Host components in hepatitis B antigen

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dc.contributor.authorBurrell, C.J.
dc.date.issued1975
dc.description.abstractPurified [125I]-labelled 20 to 25 nm hepatitis B antigen particles were found to give low affinity immunoprecipitation reactions with antisera to several normal serum components, which were immunologically distinct from the reaction due to the classical hepatitis B antigen surface determinant. These additional antigenic determinants were acid-stable and tightly bound to the particles; they could not be released by treatment with Tween 80 or ether, but were removed by protease digestion with the preservation of particle integrity. It was not possible to distinguish whether they were due to the presence of trace amounts of partly denatured serum components, or to a weak cross-reaction with antigens present in normal serum. The implications of this finding for hepatitis B antigen and antibody detection in sensititive assays are discussed. No evidence was found for native antigenic material, present in normal serum or normal liver cells, being integral to the structure of these particles.
dc.description.statementofresponsibilityC. J. Burrell
dc.identifier.citationJournal of General Virology, 1975; 27(2):117-126
dc.identifier.doi10.1099/0022-1317-27-2-117
dc.identifier.issn0022-1317
dc.identifier.issn1465-2099
dc.identifier.orcidBurrell, C.J. [0000-0002-4020-349X]
dc.identifier.urihttp://hdl.handle.net/2440/86601
dc.language.isoen
dc.publisherSociety for General Microbiology
dc.rightsCopyright status unknown
dc.source.urihttps://doi.org/10.1099/0022-1317-27-2-117
dc.subjectHumans
dc.subjectIodine Radioisotopes
dc.subjectPolysorbates
dc.subjectBromelains
dc.subjectTrypsin
dc.subjectLipoproteins
dc.subjectSerum Albumin
dc.subjectImmunoglobulin G
dc.subjectImmune Sera
dc.subjectHepatitis B Antigens
dc.subjectEpitopes
dc.subjectRadioimmunoassay
dc.subjectImmunodiffusion
dc.subjectCross Reactions
dc.titleHost components in hepatitis B antigen
dc.typeJournal article
pubs.publication-statusPublished

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