Microsatellite markers in the Australian desert plant, Solanum centrale (Solanaceae)

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dc.contributor.authorWaycott, M.
dc.contributor.authorJones, B.L.
dc.contributor.authorVan Dijk, J.K.
dc.contributor.authorRobson, H.L.
dc.contributor.authorCalladine, A.
dc.date.issued2011
dc.description.abstractPremise of the study: The first microsatellite primers were developed for Solanum centrale, a native Australian species used in the bush foods industry. Existing markers developed for other Solanum species were also amplified. Methods and Results: Using an enrichment cloning protocol, seven novel markers were developed, and 48 existing markers from other Solanum species were tested, resulting in the characterization of a set of six highly polymorphic co-dominant loci for use in S. centrale. Microsatellite screening revealed polyploidy. Among the six highly polymorphic loci, allelic diversity ranged from 7 to 14. Conclusions: These markers will be useful for investigating genetic diversity and as a simple way of estimating ploidy of wild populations.
dc.description.statementofresponsibilityMichelle Waycott, Brendan L. Jones, Jent Kornelis Van Dijk, Heather L. A. Robson and Ainsley Calladine
dc.identifier.citationAmerican Journal of Botany, 2011; 98(4):e81-e83
dc.identifier.doi10.3732/ajb.1000356
dc.identifier.issn0002-9122
dc.identifier.issn1537-2197
dc.identifier.orcidWaycott, M. [0000-0002-0822-0564]
dc.identifier.orcidVan Dijk, J.K. [0000-0002-6521-2843]
dc.identifier.orcidCalladine, A. [0000-0003-4724-8110]
dc.identifier.urihttp://hdl.handle.net/2440/87626
dc.language.isoen
dc.publisherBotanical Society of America
dc.rights© 2011 Botanical Society of America
dc.source.urihttps://doi.org/10.3732/ajb.1000356
dc.subjectmicrosatellite; polyploidy; Solanum centrale
dc.titleMicrosatellite markers in the Australian desert plant, Solanum centrale (Solanaceae)
dc.typeJournal article
pubs.publication-statusPublished

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