Investigations into the biodegradation of microcystin-LR in wastewaters

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dc.contributor.authorHo, L.
dc.contributor.authorHoefel, D.
dc.contributor.authorPalazot, S.
dc.contributor.authorSawade, E.
dc.contributor.authorNewcombe, G.
dc.contributor.authorSaint, C.
dc.contributor.authorBrookes, J.
dc.date.issued2010
dc.description.abstractMicrocystins are potent hepatotoxins that can be produced by cyanobacteria. These organisms can proliferate in wastewaters due to a number of factors including high concentrations of nutrients for growth. As treated wastewaters are now being considered as supplementary drinking water sources, in addition to their frequent use for irrigated agriculture, it is imperative that these wastewaters are free of toxins such as microcystins. This study investigated the potential for biodegradation of microcystin-LR (MCLR) in wastewaters through a biological sand filtration experiment and in static batch reactor experiments. MCLR was effectively removed at a range of concentrations and at various temperatures, with degradation attributed to the action of microorganisms indigenous to the wastewaters. No hepatotoxic by-products were detected following the degradation of MCLR as determined by a protein phosphatase inhibition assay. Using TaqMan polymerase chain reaction, the first gene involved in bacterial degradation of MCLR (mlrA) was detected and the responsible bacteria shown to increase with the amount of MCLR being degraded. This finding suggested that the degradation of MCLR was dependent upon the abundance of MCLR-degrading organisms present within the wastewater, and that MCLR may provide bacteria with a significant carbon source for proliferation; in turn increasing MCLR removal.
dc.description.statementofresponsibilityLionel Ho, Daniel Hoefel, Sebastien Palazot, Emma Sawade, Gayle Newcombe, Christopher P. Saint, Justin D. Brookes
dc.identifier.citationJournal of Hazardous Materials, 2010; 180(1-3):628-633
dc.identifier.doi10.1016/j.jhazmat.2010.04.081
dc.identifier.issn0304-3894
dc.identifier.issn1873-3336
dc.identifier.orcidBrookes, J. [0000-0001-8408-9142]
dc.identifier.urihttp://hdl.handle.net/2440/61451
dc.language.isoen
dc.publisherElsevier Science BV
dc.rightsCopyright © 2010 Elsevier B.V. All rights reserved.
dc.source.urihttps://doi.org/10.1016/j.jhazmat.2010.04.081
dc.subjectBiodegradation
dc.subjectMicrocystin
dc.subjectmlrA gene
dc.subjectPolymerase chain reaction (PCR)
dc.subjectProtein phosphatase 2A (PP2A) assay
dc.titleInvestigations into the biodegradation of microcystin-LR in wastewaters
dc.typeJournal article
pubs.publication-statusPublished

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