Enhanced Bioactivity of Enzyme/MOF Biocomposite via Host Framework Engineering

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dc.contributor.authorLiang, W.
dc.contributor.authorFlint, K.
dc.contributor.authorYao, Y.
dc.contributor.authorWu, J.
dc.contributor.authorWang, L.
dc.contributor.authorDoonan, C.
dc.contributor.authorHuang, J.
dc.date.issued2023
dc.description.abstractThis study reports the successful development of a sustainable synthesis protocol for a phase-pure metal azolate framework (MAF-6) and its application in enzyme immobilization. An esterase@MAF-6 biocomposite was synthesized, and its catalytic performance was compared with that of esterase@ZIF-8 and esterase@ZIF-90 in transesterification reactions. Esterase@MAF-6, with its large pore aperture, showed superior enzymatic performance compared to esterase@ZIF-8 and esterase@ZIF-90 in catalyzing transesterification reactions using both n-propanol and benzyl alcohol as reactants. The hydrophobic nature of the MAF-6 platform was shown to activate the immobilized esterase into its open-lid conformation, which exhibited a 1.5- and 4-times enzymatic activity as compared to free esterase in catalyzing transesterification reaction using n-propanol and benzyl alcohol, respectively. The present work offers insights into the potential of MAF-6 as a promising matrix for enzyme immobilization and highlights the need to explore MOF matrices with expanded pore apertures to broaden their practical applications in biocatalysis.
dc.description.statementofresponsibilityWeibin Liang, Kate Flint, Yuchen Yao, Jiacheng Wu, Lizhuo Wang, Christian Doonan, and Jun Huang
dc.identifier.citationJournal of the American Chemical Society, 2023; 145(37):20365-20374
dc.identifier.doi10.1021/jacs.3c05488
dc.identifier.issn0002-7863
dc.identifier.issn1520-5126
dc.identifier.orcidFlint, K. [0000-0002-9043-8369]
dc.identifier.urihttps://hdl.handle.net/2440/139571
dc.language.isoen
dc.publisherACS Publications
dc.relation.granthttp://purl.org/au-research/grants/arc/DE220100583
dc.relation.granthttp://purl.org/au-research/grants/arc/DP220102851
dc.rights© 2023 American Chemical Society
dc.source.urihttps://doi.org/10.1021/jacs.3c05488
dc.subjectBiopolymers; encapsulation; metal organic frameworks; peptides and proteins; transesterification
dc.titleEnhanced Bioactivity of Enzyme/MOF Biocomposite via Host Framework Engineering
dc.typeJournal article
pubs.publication-statusPublished

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