The Drosophila melanogaster Apaf-1 homologue ARK is required for most, but not all, programmed cell death

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dc.contributor.authorMills, K.
dc.contributor.authorDaish, T.
dc.contributor.authorHarvey, K.
dc.contributor.authorPfleger, C.
dc.contributor.authorHariharan, I.
dc.contributor.authorKumar, S.
dc.date.issued2006
dc.description© The Rockefeller University Press
dc.description.abstractThe Apaf-1 protein is essential for cytochrome c–mediated caspase-9 activation in the intrinsic mammalian pathway of apoptosis. Although Apaf-1 is the only known mammalian homologue of the Caenorhabditis elegans CED-4 protein, the deficiency of apaf-1 in cells or in mice results in a limited cell survival phenotype, suggesting that alternative mechanisms of caspase activation and apoptosis exist in mammals. In Drosophila melanogaster, the only Apaf-1/CED-4 homologue, ARK, is required for the activation of the caspase-9/CED-3–like caspase DRONC. Using specific mutants that are deficient for ark function, we demonstrate that ARK is essential for most programmed cell death (PCD) during D. melanogaster development, as well as for radiation-induced apoptosis. ark mutant embryos have extra cells, and tissues such as brain lobes and wing discs are enlarged. These tissues from ark mutant larvae lack detectable PCD. During metamorphosis, larval salivary gland removal was severely delayed in ark mutants. However, PCD occurred normally in the larval midgut, suggesting that ARK-independent cell death pathways also exist in D. melanogaster.
dc.description.statementofresponsibilityKathryn Mills, Tasman Daish, Kieran F. Harvey, Cathie M. Pfleger, Iswar K. Hariharan, and Sharad Kumar
dc.identifier.citationJournal of Cell Biology, 2006; 172(6):809-815
dc.identifier.doi10.1083/jcb.200512126
dc.identifier.issn0021-9525
dc.identifier.issn0021-9525
dc.identifier.orcidKumar, S. [0000-0001-7126-9814]
dc.identifier.urihttp://hdl.handle.net/2440/23358
dc.language.isoen
dc.publisherRockefeller Univ Press
dc.source.urihttps://doi.org/10.1083/jcb.200512126
dc.subjectSalivary Glands
dc.subjectEmbryo, Nonmammalian
dc.subjectAnimals
dc.subjectDrosophila melanogaster
dc.subjectDrosophila Proteins
dc.subjectEvolution, Molecular
dc.subjectSignal Transduction
dc.subjectApoptosis
dc.subjectGene Expression Regulation, Developmental
dc.subjectMetamorphosis, Biological
dc.subjectLarva
dc.subjectMutation
dc.subjectGenes, Lethal
dc.subjectRadiation, Ionizing
dc.titleThe Drosophila melanogaster Apaf-1 homologue ARK is required for most, but not all, programmed cell death
dc.typeJournal article
pubs.publication-statusPublished

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