Worldwide distribution and environmental origin of the Adelaide imipenemase (AIM-1), a potent carbapenemase in Pseudomonas aeruginosa

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dc.contributor.authorAmsalu, A.
dc.contributor.authorSapula, S.A.
dc.contributor.authorWhittall, J.J.
dc.contributor.authorHart, B.J.
dc.contributor.authorBell, J.M.
dc.contributor.authorTurnidge, J.
dc.contributor.authorVenter, H.
dc.date.issued2021
dc.descriptionPublished 17 December 2021
dc.description.abstractCarbapenems are potent broad-spectrum β-lactam antibiotics reserved for the treatment of serious infections caused by multidrug-resistant bacteria such as Pseudomonas aeruginosa. The surge in P. aeruginosa resistant to carbapenems is an urgent threat, as very few treatment options remain. Resistance to carbapenems is predominantly due to the presence of carbapenemase enzymes. The assessment of 147 P. aeruginosa isolates revealed that 32 isolates were carbapenem non-wild-type. These isolates were screened for carbapenem resistance genes using PCR. One isolate from wastewater contained the Adelaide imipenemase gene (bla AIM-1) and was compared phenotypically with a highly carbapenem-resistant clinical isolate containing the bla AIM-1 gene. A further investigation of wastewater samples from various local healthcare and non-healthcare sources as well as river water, using probe-based qPCR, revealed the presence of the bla AIM-1 gene in all the samples analysed. The widespread occurrence of bla AIM-1 throughout Adelaide hinted at the possibility of more generally extensive spread of this gene than originally thought. A blast search revealed the presence of the bla AIM-1 gene in Asia, North America and Europe. To elucidate the identity of the organism(s) carrying the bla AIM-1 gene, shotgun metagenomic sequencing was conducted on three wastewater samples from different locations. Comparison of these nucleotide sequences with a whole-genome sequence of a P. aeruginosa isolate revealed that, unlike the genetic environment and arrangement in P. aeruginosa, the bla AIM-1 gene was not carried as part of any mobile genetic elements. A phylogenetic tree constructed with the deduced amino acid sequences of AIM-1 suggested that the potential origin of the bla AIM-1 gene in P. aeruginosa might be the non-pathogenic environmental organism, Pseudoxanthomonas mexicana.
dc.description.statementofresponsibilityAnteneh Amsalu, Sylvia A. Sapula, Jonathan J. Whittall, Bradley J. Hart, Jan M. Bell, John Turnidge and Henrietta Venter
dc.identifier.citationMicrobial Genomics, 2021; 7(12):000715-1-000715-11
dc.identifier.doi10.1099/mgen.0.000715
dc.identifier.issn2057-5858
dc.identifier.issn2057-5858
dc.identifier.orcidTurnidge, J. [0000-0003-4240-5578]
dc.identifier.urihttps://hdl.handle.net/2440/136530
dc.language.isoen
dc.publisherMicrobiology Society
dc.relation.granthttp://purl.org/au-research/grants/nhmrc/1147538
dc.rights© 2021 The Authors. This is an open-access article distributed under the terms of the Creative Commons Attribution License.
dc.source.urihttps://doi.org/10.1099/mgen.0.000715
dc.subjectAdelaide imipenemase (AIM-1)
dc.subjectantimicrobial resistance
dc.subjectcarbapenem resistance
dc.subjectPseudomonas aeruginosa
dc.subjectPseudoxanthomonas mexicana
dc.subject.meshAnimals
dc.subject.meshHumans
dc.subject.meshPseudomonas aeruginosa
dc.subject.meshCarbapenems
dc.subject.meshbeta-Lactamases
dc.subject.meshBacterial Proteins
dc.subject.meshMicrobial Sensitivity Tests
dc.subject.meshPhylogeny
dc.subject.meshDrug Resistance, Bacterial
dc.subject.meshAmino Acid Sequence
dc.subject.meshNorth America
dc.subject.meshAsia
dc.subject.meshEurope
dc.subject.meshPhylogeography
dc.subject.meshHigh-Throughput Nucleotide Sequencing
dc.subject.meshWaste Water
dc.subject.meshWhole Genome Sequencing
dc.titleWorldwide distribution and environmental origin of the Adelaide imipenemase (AIM-1), a potent carbapenemase in Pseudomonas aeruginosa
dc.typeJournal article
pubs.publication-statusPublished

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