Calorie restriction has no effect on bone marrow tumour burden in a Vk*MYC transplant model of multiple myeloma
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Date
2022
Authors
Bradey, A.L.
Fitter, S.
Duggan, J.
Wilczek, V.
Williams, C.M.D.
Cheney, E.A.
Noll, J.E.
Tangseefa, P.
Panagopoulos, V.
Zannettino, A.C.W.
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Journal article
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Scientific Reports, 2022; 12(1):1-15
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Alanah L. Bradey, Stephen Fitter, Jvaughn Duggan, VickiWilczek, Connor M. D. Williams, EmmaAJ. Cheney, Jacqueline E. Noll, PawanratTangseefa, Vasilios Panagopoulos, Andrew C. W. Zannettino
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Abstract
Multiple myeloma (MM) is an incurable haematological malignancy, caused by the uncontrolled proliferation of plasma cells within the bone marrow (BM). Obesity is a known risk factor for MM, however, few studies have investigated the potential of dietary intervention to prevent MM progression. Calorie restriction (CR) is associated with many health benefits including reduced cancer incidence and progression. To investigate if CR could reduce MM progression, dietary regimes [30% CR, normal chow diet (NCD), or high fat diet (HFD)] were initiated in C57BL/6J mice. Diet-induced changes were assessed, followed by inoculation of mice with Vk*MYC MM cells (Vk14451-GFP) at 16 weeks of age. Tumour progression was monitored by serum paraprotein, and at endpoint, BM and splenic tumour burden was analysed by flow cytometry. 30% CR promoted weight loss, improved glucose tolerance, increased BM adiposity and elevated serum adiponectin compared to NCD-fed mice. Despite these metabolic changes, CR had no significant effect on serum paraprotein levels. Furthermore, endpoint analysis found that dietary changes were insufficient to affect BM tumour burden, however, HFD resulted in an average two-fold increase in splenic tumour burden. Overall, these findings suggest diet-induced BM changes may not be key drivers of MM progression in the Vk14451-GFP transplant model of myeloma.
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Data source: Supplementary information, https://doi.org/10.1038/s41598-022-17403-9
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© The Author(s) 2022. This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.