Enhanced capacity of n-3 fatty acid-enriched macrophages to oxidise low density lipoproteins: Mechanisms and effects of antioxidant vitamins

Simple item page
dc.contributor.authorSuzakawa, M.
dc.contributor.authorAbbey, M.
dc.contributor.authorClifton, P.
dc.contributor.authorNestel, P.
dc.date.issued1996
dc.description.abstractWe have investigated possible mechanisms by which n-3 fatty acid-enriched macrophages enhance the oxidation of low density lipoprotein (LDL), and the ability of antioxidant vitamins to prevent this. Macrophages were enriched with n-3 fatty acids (eicosapentaenoic acid, docosapentaenoic acid and docosahexaenoic acid) following incubation with fish oil. These macrophages produced large amount of TBARS in medium containing metals, and showed enhanced capacity to oxidize LDL (3-4 fold increase compared to control cells) and to accumulate the modified LDL. 5,8,11,14-eicosatetraynoic acid (ETYA, 15-lipoxygenase inhibitor) and superoxide dismutase (SOD) did not inhibit the enhanced capacity of n-3 fatty acid-enriched cells to oxidize LDL. However antioxidants, (vitamin E-enriched macrophages or vitamin C in the medium), inhibited this enhanced capacity. Medium conditioned by n-3 fatty acid-enriched cells had pro-oxidant effects on metal-initiated LDL oxidation. We conclude that n-3 fatty acid-enriched macrophages display increased oxidant capacity which is not inhibited by ETYA or SOD, and that antioxidant vitamins inhibit the enhanced capacity to oxidize LDL.
dc.identifier.citationAtherosclerosis, 1996; 124(2):157-169
dc.identifier.doi10.1016/0021-9150(96)05818-2
dc.identifier.issn0021-9150
dc.identifier.issn1879-1484
dc.identifier.orcidClifton, P. [0000-0002-6411-626X]
dc.identifier.urihttp://hdl.handle.net/2440/11731
dc.language.isoen
dc.publisherELSEVIER SCI IRELAND LTD
dc.source.urihttps://doi.org/10.1016/0021-9150(96)05818-2
dc.subjectMacrophages
dc.subjectHumans
dc.subjectArteriosclerosis
dc.subjectThiobarbituric Acid Reactive Substances
dc.subjectSuperoxide Dismutase
dc.subjectFatty Acids, Omega-3
dc.subject5,8,11,14-Eicosatetraynoic Acid
dc.subjectLipoproteins, LDL
dc.subjectAntioxidants
dc.subjectUltracentrifugation
dc.subjectChromatography, Gas
dc.subjectCell Culture Techniques
dc.subjectLipid Peroxidation
dc.titleEnhanced capacity of n-3 fatty acid-enriched macrophages to oxidise low density lipoproteins: Mechanisms and effects of antioxidant vitamins
dc.typeJournal article
pubs.publication-statusPublished

Files

Collections

Physiology publications