A chimeric GB virus B encoding the hepatitis C virus hypervariable region 1 is infectious in vivo

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dc.contributor.authorHaqshenas, G.
dc.contributor.authorDong, X.
dc.contributor.authorNetter, H.
dc.contributor.authorTorresi, J.
dc.contributor.authorGowans, E.
dc.date.issued2007
dc.description.abstractTwo GB virus B (GBV-B) chimeric genomes, GBV-HVR and GBV-HVRh (with a hinge), containing the coding region of the immunodominant hypervariable region 1 (HVR1) of the E2 envelope protein of Hepatitis C virus (HCV) were constructed. Immunoblot analysis confirmed that HVR1 was anchored to the GBV-B E2 protein. To investigate the replication competence and in vivo stability of in vitro-generated chimeric RNA transcripts, two naïve marmosets were inoculated intrahepatically with the transcripts. The GBV-HVR chimeric genome was detectable for 2 weeks post-inoculation (p.i.), whereas GBV-HVRh reverted to wild type 1 week p.i. Sequencing analysis of the HVR1 and flanking regions from GBV-HVR RNA isolated from marmoset serum demonstrated that the HVR1 insert remained unaltered in the GBV-HVR chimera for 2 weeks. Inoculation of a naïve marmoset with serum collected at 1 week p.i. also resulted in viraemia and confirmed that the serum contained infectious particles. All animals cleared the infection by 3 weeks p.i. and remained negative for the remaining weeks. The chimera may prove useful for the in vivo examination of any HCV HVR1-based vaccine candidates.
dc.description.statementofresponsibilityG. Haqshenas, X. Dong, H. Netter, J. Torresi and E. J. Gowans
dc.identifier.citationJournal of General Virology, 2007; 88(3):895-902
dc.identifier.doi10.1099/vir.0.82467-0
dc.identifier.issn0022-1317
dc.identifier.issn1465-2099
dc.identifier.orcidGowans, E. [0000-0002-4274-8311]
dc.identifier.urihttp://hdl.handle.net/2440/64433
dc.language.isoen
dc.publisherSoc General Microbiology
dc.rights© 2007 SGM
dc.source.urihttps://doi.org/10.1099/vir.0.82467-0
dc.subjectSerum
dc.subjectAnimals
dc.subjectCallithrix
dc.subjectGB virus B
dc.subjectHepacivirus
dc.subjectViremia
dc.subjectFlaviviridae Infections
dc.subjectViral Proteins
dc.subjectRNA, Viral
dc.subjectImmunoblotting
dc.subjectSequence Analysis, DNA
dc.subjectRecombination, Genetic
dc.subjectAmino Acid Sequence
dc.subjectBase Sequence
dc.subjectGenome, Viral
dc.subjectFemale
dc.titleA chimeric GB virus B encoding the hepatitis C virus hypervariable region 1 is infectious in vivo
dc.typeJournal article
pubs.publication-statusPublished

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