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Carboxymethyl chitosan/gelatin/hydroxyapatite biomimetic cryogels for bone regeneration

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dc.contributor.authorAsadi, B.
dc.contributor.authorMirzadeh, H.
dc.contributor.authorOlov, N.
dc.contributor.authorSamadikuchaksaraei, A.
dc.contributor.authorKheirbakhsh, R.
dc.contributor.authorMoradi, R.
dc.contributor.authorAmanpour, S.
dc.contributor.authorBagheri Khoulenjani, S.
dc.date.issued2023
dc.description.abstractGelation at a temperature below the freezing point of a solvent is known as cryogelation, which has attracted attention in regenerative medicine due to its improved mechanical and structural properties. This study focuses on using cryogelation as a versatile method for fabricating biomimetic scaffolds with improved mechanical strength and larger pore sizes with potential for bone tissue engineering applications. Scaffolds comprising carboxymethyl chitosan/gelatin/nano-hydroxyapatite were formed through cryogelation. The effects of three parameters – namely, cross-linker concentration, mineral content and gelatin-to-polymer ratio – on physical, chemical and mechanical properties were investigated. In this work, MG63 cells were used for an in vitro assay. In addition, an in vivo assay was conducted to investigate the biocompatibility of the nanocomposite scaffolds. The results showed that all scaffolds have a porous structure with interconnected pores with a morphology similar to bone structure. Their pore size, porosity and swelling ratio decreased with an increased cross-linker concentration, while nano-hydroxyapatite had the opposite effect. X-ray diffraction results also showed that the inorganic phase retained its crystallinity in the substrate with a slight decrease in crystal size. In addition, the scaffolds showed no toxicity in either in vitro or in vivo studies. The obtained results showed that biomimetic cryogels based on carboxymethyl chitosan/gelatin/hydroxyapatite have potential for bone tissue engineering.
dc.identifier.citationBioinspired, Biomimetic and Nanobiomaterials, 2023; 12(1):1-11
dc.identifier.doi10.1680/jbibn.22.00020
dc.identifier.issn2045-9858
dc.identifier.issn2045-9866
dc.identifier.urihttps://hdl.handle.net/11541.2/44081
dc.language.isoen
dc.publisherICE Publishing
dc.relation.fundingNational Institute for Medical Research Development, Iran 942955
dc.rightsCopyright 2025 Emerald Publishing Limited
dc.source.urihttps://doi.org/10.1680/jbibn.22.00020
dc.subjectchitosan
dc.subjectgelatin
dc.subjecthydroxyapatite
dc.subjectnanocomposites
dc.subjecttissue engineering
dc.subjectUN SDG 3: Good health and well-being
dc.titleCarboxymethyl chitosan/gelatin/hydroxyapatite biomimetic cryogels for bone regeneration
dc.typeJournal article
pubs.publication-statusPublished
ror.mmsid9916946121101831

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