Isolation and Characterization of the Gene Associated with Geosmin Production in Cyanobacteria

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dc.contributor.authorGiglio, S.
dc.contributor.authorJiang, J.
dc.contributor.authorSaint, C.
dc.contributor.authorCane, D.
dc.contributor.authorMonis, P.
dc.date.issued2008
dc.description.abstractGeosmin is a secondary metabolite responsible for earthy tastes and odors in potable water supplies. Geosmin continues to be a challenge to water utility management regimes and remains one of the most common causes of consumer complaints, as the taste of “dirty” water may suggest a failed disinfection regime and that the water may be unsafe to drink. Although cyanobacteria have been reported to be largely responsible for these taste and odor events, the answer as to how or why geosmin is produced has eluded researchers. We describe here for the first time the mechanism by which geosmin is produced in a model cyanobacterium, Nostoc punctiforme PCC 73102 (ATCC 29133), which we demonstrate utilizes a single enzyme to catalyze the cyclization of farnesyl diphosphate to geosmin. Using this information, we have developed a PCR-based assay that allows the rapid detection of geosmin-producing cyanobacteria. This test may be utilized to confirm and track the emergence of taste and odor-producing cyanobacteria in any given water body and thus can be used as an early warning system by managers of water bodies that may suffer from adverse taste and odor episodes.
dc.description.statementofresponsibilitySteven Giglio, Jiaoyang Jiang, Christopher P. Saint, David E. Cane and Paul T. Monis
dc.identifier.citationEnvironmental Science and Technology, 2008; 42(21):8027-8032
dc.identifier.doi10.1021/es801465w
dc.identifier.issn0013-936X
dc.identifier.issn1520-5851
dc.identifier.urihttp://hdl.handle.net/2440/52186
dc.language.isoen
dc.publisherAmer Chemical Soc
dc.source.urihttps://doi.org/10.1021/es801465w
dc.subjectNostoc
dc.subjectPolyisoprenyl Phosphates
dc.subjectSesquiterpenes
dc.subjectNaphthols
dc.subjectBacterial Proteins
dc.subjectDNA, Bacterial
dc.subjectPolymerase Chain Reaction
dc.subjectSequence Alignment
dc.subjectEnvironmental Microbiology
dc.subjectAmino Acid Sequence
dc.subjectNucleic Acid Denaturation
dc.subjectCyclization
dc.subjectGenes, Bacterial
dc.subjectCatalysis
dc.subjectMolecular Sequence Data
dc.subjectGas Chromatography-Mass Spectrometry
dc.titleIsolation and Characterization of the Gene Associated with Geosmin Production in Cyanobacteria
dc.typeJournal article
pubs.publication-statusPublished

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