Covalent Protein Immobilization on 3D-Printed Microfiber Meshes for Guided Cartilage Regeneration

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dc.contributor.authorAinsworth, M.J.
dc.contributor.authorLotz, O.
dc.contributor.authorGilmour, A.
dc.contributor.authorZhang, A.
dc.contributor.authorChen, M.J.
dc.contributor.authorMcKenzie, D.R.
dc.contributor.authorBilek, M.M.M.
dc.contributor.authorMalda, J.
dc.contributor.authorAkhavan, B.
dc.contributor.authorCastilho, M.
dc.date.issued2023
dc.description.abstractCurrent biomaterial-based strategies explored to treat articular cartilage defects have failed to provide adequate physico-chemical cues in order to guide functional tissue regeneration. Here, it is hypothesized that atmospheric-pressure plasma (APPJ) treatment and melt electrowriting (MEW) will produce microfiber support structures with covalently-immobilized transforming growth factor beta-1 (TGFβ1) that can stimulate the generation of functional cartilage tissue. The effect of APPJ operational speeds to activate MEW polycaprolactone meshes for immobilization of TGFβ1 is first investigated and chondrogenic differentiation and neo-cartilage production are assessed in vitro. All APPJ speeds test enhanced hydrophilicity of the meshes, with the slow treatment speed having significantly less CC/CH and more COOH than the untreated meshes. APPJ treatment increases TGFβ1 loading efficiency. Additionally, in vitro experiments highlight that APPJ-based TGFβ1 attachment to the scaffolds is more advantageous than direct supplementation within the medium. After 28 days of culture, the group with immobilized TGFβ1 has significantly increased compressive modulus (more than threefold) and higher glycosaminoglycan production (more than fivefold) than when TGFβ1 is supplied through the medium. These results demonstrate that APPJ activation allows reagent-free, covalent immobilization of TGFβ1 on microfiber meshes and, importantly, that the biofunctionalized meshes can stimulate neo-cartilage matrix formation. This opens new perspectives for guided tissue regeneration.
dc.description.statementofresponsibilityMadison J. Ainsworth, Oliver Lotz, Aaron Gilmour, Anyu Zhang, Michael J. Chen, David R. McKenzie, Marcela M.M. Bilek, Jos Malda, Behnam Akhavan, and Miguel Castilho
dc.identifier.citationAdvanced Functional Materials, 2023; 33(2):1-14
dc.identifier.doi10.1002/adfm.202206583
dc.identifier.issn1616-301X
dc.identifier.issn1616-3028
dc.identifier.urihttps://hdl.handle.net/2440/139205
dc.language.isoen
dc.publisherWiley
dc.relation.granthttp://purl.org/au-research/grants/arc/FL190100216
dc.relation.granthttp://purl.org/au-research/grants/arc/DP190103507
dc.relation.granthttp://purl.org/au-research/grants/arc/DE210100662
dc.rights© 2022 The Authors. Advanced Functional Materials published by Wiley-VCH GmbH. This is an open access article under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.
dc.source.urihttps://doi.org/10.1002/adfm.202206583
dc.subjectatmospheric-pressure plasma; cartilage; melt electrowriting; protein immobilization; stem cell differentiation; technology convergence; transforming growth factor beta
dc.titleCovalent Protein Immobilization on 3D-Printed Microfiber Meshes for Guided Cartilage Regeneration
dc.typeJournal article
pubs.publication-statusPublished

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