Premature dyad separation in meiosis II is the major segregation error with maternal age in mouse oocytes

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dc.contributor.authorYun, Y.
dc.contributor.authorLane, S.
dc.contributor.authorJones, K.
dc.date.issued2014
dc.description.abstractAs women get older their oocytes become susceptible to chromosome mis-segregation. This generates aneuploid embryos, leading to increased infertility and birth defects. Here we examined the provenance of aneuploidy by tracking chromosomes and their kinetochores in oocytes from young and aged mice. Changes consistent with chromosome cohesion deterioration were found with age, including increased interkinetochore distance and loss of the centromeric protector of cohesion SGO2 in metaphase II arrested (metII) eggs, as well as a rise in the number of weakly attached bivalents in meiosis I (MI) and lagging chromosomes at anaphase I. However, there were no MI errors in congression or biorientation. Instead, premature separation of dyads in meiosis II was the major segregation defect in aged eggs and these were associated with very low levels of SGO2. These data show that although considerable cohesion loss occurs during MI, its consequences are observed during meiosis II, when centromeric cohesion is needed to maintain dyad integrity.
dc.description.statementofresponsibilityYan Yun, Simon I. R. Lane and Keith T. Jones
dc.identifier.citationDevelopment, 2014; 141(1):199-208
dc.identifier.doi10.1242/dev.100206
dc.identifier.issn0950-1991
dc.identifier.issn1477-9129
dc.identifier.orcidJones, K. [0000-0002-0294-0851]
dc.identifier.urihttp://hdl.handle.net/2440/115683
dc.language.isoen
dc.publisherCompany of Biologists
dc.relation.granthttp://purl.org/au-research/grants/arc/DP110100418
dc.relation.granthttp://purl.org/au-research/grants/arc/DP120100946
dc.rights© 2014. Published by The Company of Biologists Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0), which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.
dc.source.urihttps://doi.org/10.1242/dev.100206
dc.subjectImaging
dc.subjectChromosomes
dc.subjectMeiosis
dc.subjectMouse
dc.subjectOocytes
dc.titlePremature dyad separation in meiosis II is the major segregation error with maternal age in mouse oocytes
dc.typeJournal article
pubs.publication-statusPublished

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