The identification of a barley haze active protein that influences beer haze stability: The genetic basis of a barley malt haze active protein
Date
2007
Authors
Robinson, L.
Healy, P.
Stewart, D.
Eglinton, J.
Ford, C.
Evans, D.
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Journal of Cereal Science, 2007; 45(3):335-342
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Abstract
In bright beers, the formation of haze is a serious quality problem, which places limitations on the storage life of the product. Sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE) immunoblot analysis using an antiserum that was raised against a silica eluate (SE) protein fraction (obtained from silica gel, used for the colloidal stabilisation of beer), detected a range of protein bands in barley, malt, beer and haze. A polymorphism was observed in which some barley varieties contained a molecular weight (MW) approximately 12,000 band (SE +ve) while in other varieties this band was absent (SE -ve). A survey of 219 Australian and international barley varieties, including a comprehensive selection of current and past malting varieties, identified 181 varieties as SE +ve, and 38 varieties as SE -ve. Previous pilot brewing trials demonstrated that SE -ve varieties are desirable as the beer brewed from the malt of these varieties formed less haze after accelerated ageing than beers brewed using SE +ve malt varieties. The genetic basis for the absence of the SE protein was conferred by a recessive allele at a single locus. Interval mapping analysis showed that the MW approximately 12,000 band mapped to the short arm of chromosome 3H.
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Copyright © 2006 Elsevier Ltd All rights reserved.