Regulation of the gene encoding glutathione S-transferase M1 (GSTM1) by the Myb oncoprotein

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dc.contributor.authorBartley, P.
dc.contributor.authorKeough, R.
dc.contributor.authorLutwyche, J.
dc.contributor.authorGonda, T.
dc.contributor.performerSchool of Molecular and Biomedical Science
dc.date.issued2003
dc.descriptionThe definitive version can be found at http://www.nature.com/index.html
dc.description.abstractThe identification of Myb ‘target’ genes will not only aid in the understanding of how overexpression of Myb, or expression of activated forms of Myb, leads to cellular transformation but will also shed light on its role in normal cells.Using a combination of an estrogenregulated Myb-transformed cell line (ERMYB) and PCR-based subtractive hybridization, we have identified the gene (GSTM1) encoding the detoxification enzyme glutathione S-transferase M1 as being transcriptionally upregulated by Myb.Functio nal analysis of the GSTM1 promoter using reporter assays indicated that both the DNA binding and transactivation domains of Myb were required for transcriptional activation.Mutationa l analysis of consensus Myb-binding sites (MBS) in the promoter and electrophoretic mobility gel shift analysis indicated that one of the three potential MBS can bind Myb protein, and is the primary site involved in the regulation of this promoter by Myb.
dc.description.statementofresponsibilityPaul A Bartley, Rebecca A Keough, Jodi K Lutwyche and Thomas J Gonda
dc.identifier.citationOncogene, 2003; 22(48):7570-7575
dc.identifier.doi10.1038/sj.onc.1207136
dc.identifier.issn0950-9232
dc.identifier.issn1476-5594
dc.identifier.orcidGonda, T. [0000-0002-8792-3021]
dc.identifier.urihttp://hdl.handle.net/2440/9729
dc.language.isoen
dc.publisherNature Publishing Group
dc.source.urihttps://doi.org/10.1038/sj.onc.1207136
dc.subjectMyb
dc.subjecttransactivation
dc.subjectglutathione S-transferase
dc.subjectpromoter
dc.titleRegulation of the gene encoding glutathione S-transferase M1 (GSTM1) by the Myb oncoprotein
dc.typeJournal article
pubs.publication-statusPublished

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