Effects of substrate hydrophobicity/hydrophilicity on the height measurement of individual DNA molecules.
Date
2007
Authors
Wang, H.
Zhou, X.
An, H.
Guo, Y.
Sun, J.
Zhang, Y.
Hu, J.
Editors
Advisors
Journal Title
Journal ISSN
Volume Title
Type:
Journal article
Citation
Chinese Physics Letters, 2007; 24(3):644-647
Statement of Responsibility
Wang, Hua-Bin; Zhou, Xing-Fei; An, Hong-Jie; Guo, Yun-Chang; Sun, Jie-Lin; Zhang, Yi and Hu, Jun
Conference Name
Abstract
Effects of substrate hydrophobicity/hydrophilicity on height measurement of individual ds-DNA molecules are investigated with tapping mode atomic force microscopy (TMAFM) and vibrating mode scanning polarization force microscopy (VSPFM). The measured heights of ds-DNA on hydrophobic highly oriented pyrolytic graphite (HOPG) are remarkably less than those on hydrophilic bare mica and Ni²⁺ treated mica in both TMAFM and VSPFM. By analysing the results, we propose that the hydrophobicity/hydrophilicity of substrate can greatly influence the height measurement of DNA molecules.