A lineage of diploid platelet-forming cells precedes polyploid megakaryocyte formation in the mouse embryo

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dc.contributor.authorPotts, K.S.
dc.contributor.authorSargeant, T.J.
dc.contributor.authorMarkham, J.F.
dc.contributor.authorShi, W.
dc.contributor.authorBiben, C.
dc.contributor.authorJosefsson, E.C.
dc.contributor.authorWhitehead, L.W.
dc.contributor.authorRogers, K.L.
dc.contributor.authorLiakhovitskaia, A.
dc.contributor.authorSmyth, G.K.
dc.contributor.authorKile, B.T.
dc.contributor.authorMedvinsky, A.
dc.contributor.authorAlexander, W.S.
dc.contributor.authorHilton, D.J.
dc.contributor.authorTaoudi, S.
dc.date.issued2014
dc.description.abstractIn this study, we test the assumption that the hematopoietic progenitor/colony-forming cells of the embryonic yolk sac (YS), which are endowed with megakaryocytic potential, differentiate into the first platelet-forming cells in vivo. We demonstrate that from embryonic day (E) 8.5 all megakaryocyte (MK) colony-forming cells belong to the conventional hematopoietic progenitor cell (HPC) compartment. Although these cells are indeed capable of generating polyploid MKs, they are not the source of the first platelet-forming cells. We show that proplatelet formation first occurs in a unique and previously unrecognized lineage of diploid platelet-forming cells, which develop within the YS in parallel to HPCs but can be specified in the E8.5 Runx1-null embryo despite the absence of the progenitor cell lineage.
dc.description.statementofresponsibilityKathryn S. Potts, Tobias J. Sargeant, John F. Markham, Wei Shi, Christine Biben ... Benjamin T. Kile ... et al.
dc.identifier.citationBlood, 2014; 124(17):2725-2729
dc.identifier.doi10.1182/blood-2014-02-559468
dc.identifier.issn0006-4971
dc.identifier.issn1528-0020
dc.identifier.orcidKile, B.T. [0000-0002-8836-8947]
dc.identifier.urihttp://hdl.handle.net/2440/121904
dc.language.isoen
dc.publisherAmerican Society of Hematology
dc.relation.granthttp://purl.org/au-research/grants/arc/1016647
dc.relation.granthttp://purl.org/au-research/grants/nhmrc/361646
dc.rights© 2014 by The American Society of Hematology.
dc.source.urihttps://doi.org/10.1182/blood-2014-02-559468
dc.subjectMegakaryocytes
dc.titleA lineage of diploid platelet-forming cells precedes polyploid megakaryocyte formation in the mouse embryo
dc.typeJournal article
pubs.publication-statusPublished

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