Polymeric stationary phases for size exclusion chromatography: a review
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Date
2021
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Lubomirsky, E.
Khodabandeh, A.
Preis, J.
Susewind, M.
Hofe, T.
Hilder, E.F.
Arrua, R.D.
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Analytica Chimica Acta, 2021; 1151(article no. 338244):1-18
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Synthetic and natural macromolecules are commonly used in a variety of fields such as plastics, nano-medicine, biotherapeutics, drug delivery and tissue engineering. Characterising macromolecules in terms of their structural parameters (size, molar mass and distribution, architecture) is key to have a better understanding of their structure-property relationships. Size exclusion chromatography (SEC) is a commonly used technique for polymer characterization since it offers access to the determination of the size of a macromolecule, its molar mass and the molar mass distribution. Moreover, detectors that allow the determination of true molar masses, macromolecule’s architecture and the composition of co-polymers can be coupled to the chromatographic system.
Like other chromatographic techniques, the stationary phase is of paramount importance for efficient SEC separations. This review presents the basic principles for the design of stationary phases for SEC as well as synthetic methods currently used in the field. Current status of fully-porous polymeric stationary phases used in SEC is reviewed and their advantages and limitations are also discussed. Finally, the potential of polymer monoliths in SEC is also covered, highlighting the limitations this column technology could address. However, further development in the polymer structure is needed to consider this column technology in the field of macromolecule separation.
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Copyright 2021 Elsevier
Access Condition Notes: Accepted manuscript available after 1 April 2022