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Effects of intraduodenal infusion of lauric acid and L-tryptophan, alone and combined, on glucoregulatory hormones, gastric emptying and glycaemia in healthy men

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dc.contributor.authorHajishafiee, M.
dc.contributor.authorMcVeay, C.
dc.contributor.authorLange, K.
dc.contributor.authorRehfeld, J.
dc.contributor.authorHorowitz, M.
dc.contributor.authorFeinle-Bisset, C.
dc.date.issued2022
dc.description.abstractBackground and aim: In healthy men, intraduodenal administration of the fatty acid, lauric acid (‘C12’) and the amino acid, L-tryptophan (‘TRP’), at loads that individually do not affect energy intake, reduce energy intake sub- stantially when combined. C12 and TRP may also stimulate cholecystokinin and glucagon-like peptide-1 (GLP-1), which both slow gastric emptying, a key determinant of postprandial blood glucose. Accordingly, combination of C12 and TRP has the potential to reduce post-meal glycaemia more than either nutrient alone. Methods: Twelve healthy, lean men (age (mean ± SD): 28 ± 7 years) received, on 4 separate occasions, 45-min intraduodenal infusions of C12 (0.3 kcal/min), TRP (0.1 kcal/min), C12 + TRP (0.4 kcal/min), or 0.9% saline (control), in a randomised, double-blind fashion. 30 min after commencement of the infusion a mixed- nutrient drink was consumed and gastric emptying measured (13C breath-test) for 3 h. Blood samples were obtained at baseline, in response to treatments alone, and for 2 h post-drink for measurements of plasma glucose, cholecystokinin, GLP-1, C-peptide, insulin and glucagon. ‘Early’ (first 30 min) and ‘overall’ glycaemic and hormone responses were evaluated. Results: C12 + TRP and C12 delayed the rise in, but did not affect the overall glycaemic response to the drink, compared with control and TRP (all P < 0.05). C12 + TRP slowed gastric emptying compared with control and TRP (both P < 0.005), and C12 non-significantly slowed gastric emptying compared with control (P = 0.090). C12 + TRP and C12 delayed the rise in C-peptide and insulin, and also stimulated CCK and glucagon, compared with control and TRP (all P < 0.05). Only C12 + TRP stimulated early and overall GLP-1 compared with control (P < 0.05). Conclusions: In healthy men, C12 + TRP and C12, in the loads administered, had comparable effects to delay the rise in glucose following a nutrient drink, probably primarily by slowing of gastric emptying, as a result of CCK and GLP-1 stimulation, while TRP had no effect.
dc.description.statementofresponsibilityM. Hajishafiee, C. McVeay. K. Lange, J.F. Rehfeld, M. Horowitz, C. Feinle-Bisset
dc.identifier.citationMetabolism: Clinical and Experimental, 2022; 129:1-9
dc.identifier.doi10.1016/j.metabol.2022.155140
dc.identifier.issn0026-0495
dc.identifier.issn1532-8600
dc.identifier.orcidLange, K. [0000-0003-3814-8513]
dc.identifier.orcidHorowitz, M. [0000-0002-0942-0306]
dc.identifier.orcidFeinle-Bisset, C. [0000-0001-6848-0125]
dc.identifier.urihttps://hdl.handle.net/2440/134864
dc.language.isoen
dc.publisherElsevier
dc.relation.granthttp://purl.org/au-research/grants/nhmrc/1158296
dc.relation.granthttp://purl.org/au-research/grants/nhmrc/1103020
dc.rights© 2022 Elsevier Inc. All rights reserved.
dc.source.urihttps://doi.org/10.1016/j.metabol.2022.155140
dc.subjectNutrients
dc.subjectGastric emptying
dc.subjectGlycaemic control
dc.subjectIncretin hormone
dc.subjectHuman
dc.titleEffects of intraduodenal infusion of lauric acid and L-tryptophan, alone and combined, on glucoregulatory hormones, gastric emptying and glycaemia in healthy men
dc.typeJournal article
pubs.publication-statusPublished

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