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In the heat of the night - alternative pathway respiration drives thermogenesis in Philodendron bipinnatifidum

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dc.contributor.authorMiller, R.
dc.contributor.authorGrant, N.
dc.contributor.authorGiles, L.
dc.contributor.authorRibas-carbo, M.
dc.contributor.authorBerry, J.
dc.contributor.authorWatling, J.
dc.contributor.authorRobinson, S.
dc.date.issued2011
dc.descriptionArticle first published online: 30 NOV 2010
dc.description.abstractPHILODENDRON bipinnatifidum inflorescences heat up to 42°C and thermoregulate. We investigated whether they generate heat via the cytochrome oxidase pathway uncoupled by uncoupling proteins (pUCPs), or the alternative oxidase (AOX). CONTRIBUTION of AOX and pUCPs to heating in fertile (FM) and sterile (SM) male florets was determined using a combination of oxygen isotope discrimination, protein and substrate analyses. BOTH FM and SM florets thermoregulated independently for up to 30 h ex planta. In both floret types, AOX contributed > 90% of respiratory flux during peak heating. The AOX protein increased fivefold with the onset of thermogenesis in both floret types, whereas pUCP remained low throughout development. These data indicate that AOX is primarily responsible for heating, despite FM and SM florets potentially using different substrates, carbohydrates or lipids, respectively. Measurements of discrimination between O2 isotopes in strongly respiring SM florets were affected by diffusion; however, this diffusional limitation was largely overcome using elevated O2. THE first in vivo respiratory flux measurements in an arum show AOX contributes the bulk of heating in P. bipinnatifidum. Fine-scale regulation of AOX activity is post-translational. We also demonstrate that elevated O2 can aid measurement of respiratory pathway fluxes in dense tissues.
dc.description.statementofresponsibilityRebecca E. Miller, Nicole M. Grant, Larry Giles, Miquel Ribas-Carbo, Joseph A. Berry, Jennifer R. Watling and Sharon A. Robinson
dc.identifier.citationNew Phytologist, 2011; 189(4):1013-1026
dc.identifier.doi10.1111/j.1469-8137.2010.03547.x
dc.identifier.issn0028-646X
dc.identifier.issn1469-8137
dc.identifier.orcidWatling, J. [0000-0001-6305-9905]
dc.identifier.urihttp://hdl.handle.net/2440/68433
dc.language.isoen
dc.publisherBlackwell Publishing Ltd
dc.relation.granthttp://purl.org/au-research/grants/arc/DP0451617
dc.relation.granthttp://purl.org/au-research/grants/arc/DP0451617
dc.rights© 2010 The Authors. New Phytologist © 2010 New Phytologist Trust
dc.source.urihttps://doi.org/10.1111/j.1469-8137.2010.03547.x
dc.subjectAlternative oxidase
dc.subjectAraceae
dc.subjectdiffusion limitation
dc.subjectPhilodendron bipinnatifidum
dc.subjectplant thermogenesis
dc.subjectplant uncoupling proteins
dc.subjectstable isotope measurements of respiration
dc.titleIn the heat of the night - alternative pathway respiration drives thermogenesis in Philodendron bipinnatifidum
dc.typeJournal article
pubs.publication-statusPublished

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