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Protein adsorption determines pulmonary cell uptake of lipid-based nanoparticles

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dc.contributor.authorSubramaniam, S.
dc.contributor.authorJoyce, P.
dc.contributor.authorDonnellan, L.
dc.contributor.authorYoung, C.
dc.contributor.authorWignall, A.
dc.contributor.authorHoffmann, P.
dc.contributor.authorPrestidge, C.A.
dc.date.issued2023
dc.description.abstractThe inhalable administration of lipid nanoparticles is an effective strategy for localised delivery of therapeutics against various lung diseases. Of this, improved intracellular delivery of pharmaceuticals for infectious disease and cancer management is of high significance. However, the influence of lipid nanoparticle composition and structure on uptake in pulmonary cell lines, especially in the presence of biologically relevant media is poorly understood. Here, the uptake of lamellar (liposomes) versus non-lamellar (cubosomes) lipid nanoparticles in macrophages and lung epithelial cells was quantified and the influence of bronchoalveolar lavage fluid (BALF), containing native pulmonary protein and surfactant molecules is determined. Cubosome uptake in both macrophages and epithelial cells was strongly mediated by a high percentage of molecular function regulatory and binding proteins present within the protein corona. In contrast, the protein corona did not influence the uptake of liposomes in epithelial cells. In macrophages, the proteins mediated a rapid internalisation, followed by exocytosis of liposomes after 6 h incubation. These findings on the influence of biological fluid in regulating lipid nanoparticle uptake mechanisms may guide future development of optimal intracellular delivery systems for therapeutics via the pulmonary route.
dc.identifier.citationJournal of Colloid and Interface Science, 2023; 641:36-47
dc.identifier.doi10.1016/j.jcis.2023.03.048
dc.identifier.issn0021-9797
dc.identifier.issn1095-7103
dc.identifier.orcidHoffmann, P. [0000-0002-6573-983X]
dc.identifier.urihttps://hdl.handle.net/11541.2/33493
dc.language.isoen
dc.publisherACADEMIC PRESS INC ELSEVIER SCIENCE
dc.relation.fundingState and Federal Governments
dc.rightsCopyright 2023 The author(s), published Open Access by Elsevier (https://creativecommons.org/licenses/by-nc/4.0/)
dc.source.urihttps://doi.org/10.1016/j.jcis.2023.03.048
dc.subjectLung
dc.subjectLipids
dc.subjectProteins
dc.subjectLiposomes
dc.subjectAdsorption
dc.subjectNanoparticles
dc.subjectProtein Corona
dc.titleProtein adsorption determines pulmonary cell uptake of lipid-based nanoparticles
dc.typeJournal article
pubs.publication-statusPublished
ror.fileinfo12260888640001831 13260888630001831 Protein adsorption determines pulmonary cell uptake of lipid-based nanoparticles
ror.mmsid9916721797201831

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