A method to isolate and purify human bone marrow stromal stem cells
Date
2008
Authors
Gronthos, S.
Zannettino, A.
Editors
Prockop, D.
Phinney, D.
Bunnell, B.
Phinney, D.
Bunnell, B.
Advisors
Journal Title
Journal ISSN
Volume Title
Type:
Book chapter
Citation
Mesenchymal Stem Cells: Methods and Protocols, 2008 / Prockop, D., Phinney, D., Bunnell, B. (ed./s), vol.449, pp.45-57
Statement of Responsibility
Stan Gronthos and Andrew C. W. Zannettino
Conference Name
Abstract
The STRO-1 antibody can be used as a single reagent to isolate human bone marrow stromal stem cells (BMSSC), owing to its restricted specificity to a cell surface molecule expressed by clonogenic BMSSC, with little or no reactivity to hematopoietic stem/progenitor populations or mature stromal elements. The present protocol uses a combination of two different immunoselection methodologies in an attempt to generate highly purified preparations of BMSSC. This process involves the initial isolation of a minor subpopulation of bone marrow mononuclear cells (approx 10%) expressing the STRO-1 antigen, by means of magnetic activated cell sorting. Dual-color fluorescence activated cell sorting is then used as a secondary step to further purify the rare STRO-1bright expressing fraction that contains all of the colony-forming BMSSC, based on their co-expression of a secondary cell surface marker, CD106 (VCAM-1).
School/Discipline
Dissertation Note
Provenance
Description
The original publication can be found at www.springerlink.com