Novel polyethylene glycol derivative suitable for the preparation of mono-PEGylated protein

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dc.contributor.authorYun, Q.
dc.contributor.authorChen, T.
dc.contributor.authorZhang, G.
dc.contributor.authorBi, J.
dc.contributor.authorMa, G.
dc.contributor.authorSu, Z.
dc.date.issued2005
dc.descriptionThe definitive version may be found at www.springerlink.com
dc.description.abstractA novel methoxypolyethylene glycol (mPEG) derivative, containing a reactive group of 1-methyl pyridinium toluene-4-sulfonate, was synthesized and characterized. The mPEG derivative was successfully conjugated with two proteins: recombinant human granulocyte-colony stimulating factor (rhG-CSF) and consensus interferon (C-IFN). Homogeneous mono-PEGylated proteins were obtained which were identified by high performance size-exclusion chromatography and MALDI-TOF mass spectrometry. The biological activities of the mono-PEGylated rhG-CSF and the mono-PEGylated C-IFN were maintained at 90% and 88%, respectively.
dc.description.statementofresponsibilityQiang Yun, Ting Chen, Guifeng Zhang, Jingxiu Bi, Guanghui Ma and Zhiguo Su
dc.identifier.citationBiotechnology Letters, 2005; 27(3):213-217
dc.identifier.doi10.1007/s10529-004-8354-7
dc.identifier.issn0141-5492
dc.identifier.issn1573-6776
dc.identifier.orcidBi, J. [0000-0001-7056-8572]
dc.identifier.urihttp://hdl.handle.net/2440/46419
dc.language.isoen
dc.publisherKluwer Academic Publ
dc.rights© Springer
dc.source.urihttp://www.springerlink.com/content/v7l8l16385257262/
dc.subjectconsensus interferon
dc.subjectgranulocyte-colony stimulating factor
dc.subjectPEGylation
dc.subjectpolyethylene glycol
dc.titleNovel polyethylene glycol derivative suitable for the preparation of mono-PEGylated protein
dc.typeJournal article
pubs.publication-statusPublished

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