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Soybean SAT1 (Symbiotic Ammonium Transporter 1) encodes a bHLH transcription factor involved in nodule growth and NH₄⁺ transport

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dc.contributor.authorChiasson, D.
dc.contributor.authorLoughlin, P.
dc.contributor.authorMazurkiewicz, D.
dc.contributor.authorMohammadidehcheshmeh, M.
dc.contributor.authorFedorova, E.
dc.contributor.authorOkamoto, M.
dc.contributor.authorMcLean, E.
dc.contributor.authorGlass, A.
dc.contributor.authorSmith, S.
dc.contributor.authorBisseling, T.
dc.contributor.authorTyerman, S.
dc.contributor.authorDay, D.
dc.contributor.authorKaiser, B.
dc.date.issued2014
dc.description.abstractGlycine max symbiotic ammonium transporter 1 was first documented as a putative ammonium (NH4(+)) channel localized to the symbiosome membrane of soybean root nodules. We show that Glycine max symbiotic ammonium transporter 1 is actually a membrane-localized basic helix-loop-helix (bHLH) DNA-binding transcription factor now renamed Glycine max bHLH membrane 1 (GmbHLHm1). In yeast, GmbHLHm1 enters the nucleus and transcriptionally activates a unique plasma membrane NH4(+) channel Saccharomyces cerevisiae ammonium facilitator 1. Ammonium facilitator 1 homologs are present in soybean and other plant species, where they often share chromosomal microsynteny with bHLHm1 loci. GmbHLHm1 is important to the soybean rhizobium symbiosis because loss of activity results in a reduction of nodule fitness and growth. Transcriptional changes in nodules highlight downstream signaling pathways involving circadian clock regulation, nutrient transport, hormone signaling, and cell wall modification. Collectively, these results show that GmbHLHm1 influences nodule development and activity and is linked to a novel mechanism for NH4(+) transport common to both yeast and plants.
dc.description.statementofresponsibilityDavid M. Chiasson, Patrick C. Loughlin, Danielle Mazurkiewicz, Manijeh Mohammadidehcheshmeh, Elena E. Fedorova, Mamoru Okamoto, Elizabeth McLean, Anthony D. M. Glass, Sally E. Smith, Ton Bisseling, Stephen D. Tyerman, David A. Day, and Brent N. Kaiser
dc.identifier.citationProceedings of the National Academy of Sciences of the United States of America, 2014; 111(13):4814-4819
dc.identifier.doi10.1073/pnas.1312801111
dc.identifier.issn0027-8424
dc.identifier.issn1091-6490
dc.identifier.orcidOkamoto, M. [0000-0002-2989-607X]
dc.identifier.orcidTyerman, S. [0000-0003-2455-1643]
dc.identifier.urihttp://hdl.handle.net/2440/88386
dc.language.isoen
dc.publisherNational Academy of Sciences
dc.relation.granthttp://purl.org/au-research/grants/arc/DP110100245
dc.relation.granthttp://purl.org/au-research/grants/arc/DP0450577
dc.source.urihttps://doi.org/10.1073/pnas.1312801111
dc.subjectCell Membrane
dc.subjectSaccharomyces cerevisiae
dc.subjectSoybeans
dc.subjectAmmonium Compounds
dc.subjectCation Transport Proteins
dc.subjectSoybean Proteins
dc.subjectDNA, Plant
dc.subjectGene Expression Regulation, Plant
dc.subjectProtein Binding
dc.subjectBiological Transport
dc.subjectBasic Helix-Loop-Helix Leucine Zipper Transcription Factors
dc.subjectRoot Nodules, Plant
dc.titleSoybean SAT1 (Symbiotic Ammonium Transporter 1) encodes a bHLH transcription factor involved in nodule growth and NH₄⁺ transport
dc.title.alternativeSoybean SAT1 (Symbiotic Ammonium Transporter 1) encodes a bHLH transcription factor involved in nodule growth and NH(4)(+) transport
dc.typeJournal article
pubs.publication-statusPublished

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