Characterization of the EphA1 receptor tyrosine kinase: Expression in epithelial tissues

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dc.contributor.authorCoulthard, M.
dc.contributor.authorLickliter, J.
dc.contributor.authorSubanesan, N.
dc.contributor.authorChen, K.
dc.contributor.authorWebb, G.
dc.contributor.authorLowry, A.
dc.contributor.authorKoblar, S.
dc.contributor.authorBottema, C.
dc.contributor.authorBoyd, A.
dc.contributor.organisationCentre for the Molecular Genetics of Development
dc.date.issued2001
dc.description.abstractThe Eph family of receptor tyrosine kinases plays a crucial role during development and is implicated in oncogenesis. Using a partial cDNA clone of an Eph-related kinase (Esk) we isolated the complete coding region of a gene which we show to be murine EphA1 by both structural and functional criteria. The chromosomal localization is shown to be syntenic to hEphA1 and the genomic organization also shows distinct features found in the hEphA1 gene. Functionally, in keeping with findings for the human homologue, both soluble recombinant and "native" mEphA1 show preferential binding to ephrin A1. However, we also observed significant binding to other A-type ligands as has been observed for other Eph receptors. We analysed the expression of mEphA1 mRNA by in situ hybridization on tissue sections. mEphA1 was expressed in epithelial elements of skin, adult thymus, kidney and adrenal cortex. Taken together with previous Northern blotting data these results suggest that mEphA1 is expressed widely in differentiated epithelial cells.
dc.identifier.citationGrowth Factors, 2001; 18(4):303-317
dc.identifier.doi10.3109/08977190109029118
dc.identifier.issn0897-7194
dc.identifier.issn1029-2292
dc.identifier.orcidKoblar, S. [0000-0002-8667-203X]
dc.identifier.orcidBottema, C. [0000-0001-6245-0099]
dc.identifier.urihttp://hdl.handle.net/2440/13236
dc.language.isoen
dc.publisherHarwood Acad Publ GMBH
dc.source.urihttps://doi.org/10.3109/08977190109029118
dc.subjectEph
dc.subjectreceptor tyrosine kinase
dc.subjectepithelial tissue
dc.subjectgenomic organisation
dc.subjectthymus
dc.titleCharacterization of the EphA1 receptor tyrosine kinase: Expression in epithelial tissues
dc.typeJournal article
pubs.publication-statusPublished

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