Differences of histone H4 acetylation and replication timing between A and B chromosomes of Brachycome dichromosomatica

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dc.contributor.authorHouben, A.
dc.contributor.authorBelyaev, N.
dc.contributor.authorLeach, C.
dc.contributor.authorTimmis, J.
dc.date.issued1997
dc.descriptionThe original publication can be found at www.springerlink.com
dc.description.abstractDifferences are demonstrated between A (transcriptionally active) and B (transcriptionally inactive) chromosomes that are characterized by a different level of histone H4 acetylation and a different timing of DNA replication. These differences between the chromatin of A and B chromosomes were found after immunolabelling of chromsomes of Brachycome dichromosomatica with antibodies specific for different acetylated forms (lysine 5, 8, 12 and 16) of histone H4. In contrast to the A chromosomes, which are labelled brightly in their entirety, the transcriptionally inactive B chromosomes are faintly labelled with antibodies against H4Ac5 and H4Ac8. No such difference between the chromosomes is found after immunostaining with the other antibodies H4Ac12 and H4Ac16. Analysis of DNA replication timing in root-tip meristems suggests that B chromosomes are labelled late in S-phase compared with A chromosomes. After C-banding the B chromosome appeared to have a similar amount of heterochromatin to the A chromosomes.
dc.description.statementofresponsibilityAndreas Houben, Nikolai D. Belyaev, Carolyn R. Leach and Jeremy N. Timmis
dc.identifier.citationChromosome Research, 1997; 5(4):233-237
dc.identifier.doi10.1023/B:CHRO.0000032297.10876.86
dc.identifier.issn0967-3849
dc.identifier.issn1573-6849
dc.identifier.urihttp://hdl.handle.net/2440/11410
dc.language.isoen
dc.publisherRAPID SCIENCE PUBLISHERS
dc.source.urihttps://doi.org/10.1023/b:chro.0000032297.10876.86
dc.subjectacetylated histone H4
dc.subjectB chromosome
dc.subjectheterochromatin
dc.subjectplants
dc.subjectreplication
dc.titleDifferences of histone H4 acetylation and replication timing between A and B chromosomes of Brachycome dichromosomatica
dc.typeJournal article
pubs.publication-statusPublished

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