Directed differentiation of pluripotent cells to neural lineages: homogeneous formation and differentiation of a neurectoderm population

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dc.contributor.authorRathjen, Joy R.en
dc.contributor.authorHaines, Bryan Peteren
dc.contributor.authorHudson, Kathryn M.en
dc.contributor.authorNesci, Antoniettaen
dc.contributor.authorDunn, Stephanie M.en
dc.contributor.authorRathjen, Peter Daviden
dc.contributor.organisationCentre for the Molecular Genetics of Developmenten
dc.contributor.schoolSchool of Molecular and Biomedical Scienceen
dc.date.issued2002en
dc.description.abstractDuring embryogenesis the central and peripheral nervous systems arise from a neural precursor population, neurectoderm, formed during gastrulation. We demonstrate the differentiation of mouse embryonic stem cells to neurectoderm in culture, in a manner which recapitulates embryogenesis, with the sequential and homogeneous formation of primitive ectoderm, neural plate and neural tube. Formation of neurectoderm occurs in the absence of extraembryonic endoderm or mesoderm and results in a stratified epithelium of cells with morphology, gene expression and differentiation potential consistent with positionally unspecified neural tube. Differentiation of this population to homogeneous populations of neural crest or glia was also achieved. Neurectoderm formation in culture allows elucidation of signals involved in neural specification and generation of implantable cell populations for therapeutic use.en
dc.identifier.citationDevelopment, 2002; 129(11):2649-2661en
dc.identifier.issn0950-1991en
dc.identifier.urihttp://hdl.handle.net/2440/28151
dc.language.isoenen
dc.publisherCompany of Biologistsen
dc.source.urihttp://dev.biologists.org/cgi/content/abstract/129/11/2649en
dc.subjectStem cells; Neurectoderm; Cell culture; Neural cresten
dc.titleDirected differentiation of pluripotent cells to neural lineages: homogeneous formation and differentiation of a neurectoderm populationen
dc.typeJournal articleen

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