Genome-wide characterization and expression profiling of immune genes in the diamondback moth, Plutella xylostella (L.)

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dc.contributor.authorXia, X.
dc.contributor.authorYu, L.
dc.contributor.authorXue, M.
dc.contributor.authorYu, X.
dc.contributor.authorVasseur, L.
dc.contributor.authorGurr, G.
dc.contributor.authorBaxter, S.
dc.contributor.authorLin, H.
dc.contributor.authorLin, J.
dc.contributor.authorYou, M.
dc.date.issued2015
dc.description.abstractThe diamondback moth, Plutella xylostella (L.), is a destructive pest that attacks cruciferous crops worldwide. Immune responses are important for interactions between insects and pathogens and information on these underpins the development of strategies for biocontrol-based pest management. Little, however, is known about immune genes and their regulation patterns in P. xylostella. A total of 149 immune-related genes in 20 gene families were identified through comparison of P. xylostella genome with the genomes of other insects. Complete and conserved Toll, IMD and JAK-STAT signaling pathways were found in P. xylostella. Genes involved in pathogen recognition were expanded and more diversified than genes associated with intracellular signal transduction. Gene expression profiles showed that the IMD pathway may regulate expression of antimicrobial peptide (AMP) genes in the midgut, and be related to an observed down-regulation of AMPs in experimental lines of insecticide-resistant P. xylostella. A bacterial feeding study demonstrated that P. xylostella could activate different AMPs in response to bacterial infection. This study has established a framework of comprehensive expression profiles that highlight cues for immune regulation in a major pest. Our work provides a foundation for further studies on the functions of P. xylostella immune genes and mechanisms of innate immunity.
dc.description.statementofresponsibilityXiaofeng Xia, Liying Yu, Minqian Xue, Xiaoqiang Yu, Liette Vasseur, Geoff M. Gurr, Simon W. Baxter, Hailan Lin, Junhan Lin, Minsheng You
dc.identifier.citationScientific Reports, 2015; 5(1):09877-1-09877-13
dc.identifier.doi10.1038/srep09877
dc.identifier.issn2045-2322
dc.identifier.issn2045-2322
dc.identifier.orcidBaxter, S. [0000-0001-5773-6578]
dc.identifier.urihttp://hdl.handle.net/2440/94180
dc.language.isoen
dc.publisherNature
dc.rightsThis work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
dc.source.urihttps://doi.org/10.1038/srep09877
dc.subjectInnate immunity; transciptomics
dc.titleGenome-wide characterization and expression profiling of immune genes in the diamondback moth, Plutella xylostella (L.)
dc.typeJournal article
pubs.publication-statusPublished

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