Molecular and Genetic Characterization of the Capsule Biosynthesis Locus Of Streptococcus pneumoniae Type 19B

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dc.contributor.authorMorona, J.
dc.contributor.authorMorona, R.
dc.contributor.authorPaton, J.
dc.date.issued1997
dc.description.abstractWe have previously reported the nucleotide sequence of the Streptococcus pneumoniae type 19F capsular polysaccharide synthesis locus (cps19f), which consists of 15 open reading frames (ORFs) designated cps19fA to -O. Hybridization analysis indicated that close homologs for cps19fA to -H and cps19fK to -O were found in type 19B, but there were no homologs for cps19fI and -J. In this study we used long-range PCR to amplify and clone a 10.5-kb section of the S. pneumoniae type 19B capsule locus (cps19b) between cps19bH and cps19bK. This region of the cps19b locus is 4 kb larger than that in the cps19f locus and replaces cps19fI and cps19fJ with five new ORFs, designated cps19bP, -I, -Q, -R, and -J. We have proposed functions for four of the protein products, including functional homologs of Cps19fI and Cps19fJ. Transformation of a S. pneumoniae mutant containing an interrupted type 19F capsule locus with the 10.5-kb cps19b PCR product converted the recipient strain to type 19B. Southern hybridization analysis indicated that cps19bP, -I, -Q, -R, and -J are unique to type 19B and the closely related type 19C.
dc.identifier.citationJournal of Bacteriology, 1997; 179(15):4953-4958
dc.identifier.doi10.1128/jb.179.15.4953-4958.1997
dc.identifier.issn0021-9193
dc.identifier.issn1098-5530
dc.identifier.orcidMorona, J. [0000-0002-4891-4122]
dc.identifier.orcidMorona, R. [0000-0001-7009-7440]
dc.identifier.orcidPaton, J. [0000-0001-9807-5278]
dc.identifier.urihttp://hdl.handle.net/2440/11610
dc.language.isoen
dc.publisherAMER SOC MICROBIOLOGY
dc.source.urihttps://doi.org/10.1128/jb.179.15.4953-4958.1997
dc.subjectStreptococcus pneumoniae
dc.subjectBacterial Capsules
dc.subjectChromosome Mapping
dc.subjectTransformation, Bacterial
dc.subjectAmino Acid Sequence
dc.subjectBase Sequence
dc.subjectSequence Homology, Amino Acid
dc.subjectGenes, Bacterial
dc.subjectMolecular Sequence Data
dc.titleMolecular and Genetic Characterization of the Capsule Biosynthesis Locus Of Streptococcus pneumoniae Type 19B
dc.typeJournal article
pubs.publication-statusPublished

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