Possibility of combination inorganic (celite) and organic (Alginate) materials to immobilize glucoamylase enzyme
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(Published version)
Date
2011
Authors
Mai, T.H.L.
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Conference paper
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Proceedings of the 3rd AUN/SEED-NET Regional Conference on Biotechnology, as published in Vietnam Journal of Science and Technology, 2011, vol.49, iss.1A, pp.269-275
Statement of Responsibility
Huynh Ngoc Oanh, Mai Thi Hong Linh, E, inh Thi Kim Loan, Dong Thi Thanh Thu
Conference Name
The 3rd Regional Conference on Biotechnology: Bioproducts and Bioprocess (3 Mar 2011 - 4 Mar 2011 : Ha Noi, Vietnam)
Abstract
Glucoamylase (AMG) is an enzyme that is widely used in the food industry. Celite and alginate are two polymers that can immobilize enzymes rather well. With celite, under the optimal immobilization conditions (10 AMG with 1g celite (v/w), the activity of immobilized efficiency and active efficiency of immobilized AMG is 36.65% and 1.56%, respectively. Immobilization of AMG by using celite-alginate complex was observed. The result demonstrated that AMG stability was increased, and the active efficiency was 17.2%. Otherwise, after the 22nd use, the retained activity for immobilized AMG was found as 53.28%. Therefore, the celite-alginate complex has potential application in the immobilization of AMG.
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