Proteomic analysis reveals the potential involvement of xylanase from Pyrenophora teres f. teres in net form net blotch disease of barley
Date
2014
Authors
Ismail, I.
Godfrey, D.
Able, A.
Editors
Advisors
Journal Title
Journal ISSN
Volume Title
Type:
Journal article
Citation
Australasian Plant Pathology, 2014; 43(6):715-726
Statement of Responsibility
I. A. Ismail, D. Godfrey, A. J. Able
Conference Name
Abstract
The barley pathogen Pyrenophora teres f. teres (Ptt) produces proteinaceous toxins that contribute to the necrotic symptoms observed during net form net blotch (NFNB) disease. To better understand the relationship between these toxins and virulence, a proteomics approach was used to identify proteins differentially expressed in a more virulent Ptt isolate. Three proteins were identified: an endo-1,4-β-xylanase A (PttXyn11A), a cysteine hydrolase family protein (PttCHFP1) and an unknown (but conserved) secreted protein (PttSP1). PttXyn11A was homologous to a plant cell-wall degrading enzyme but also had a predicted necrosis-inducing region on the enzyme surface. PttCHFP1 showed homology to an isochorismatase, an enzyme proposed to suppress plant defence. Xylanase activity and PttXyn11A expression were greater in more virulent isolates in vitro and during the interaction respectively, suggesting that PttXyn11A plays a role in symptom development.
School/Discipline
Dissertation Note
Provenance
Description
Access Status
Rights
© Australasian Plant Pathology Society Inc. 2014