Comparative study of genes expressed from rice fungus-resistant and susceptible lines during interactions with Magnaporthe oryzae

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dc.contributor.authorShi, B.
dc.contributor.authorWang, G.
dc.date.issued2008
dc.descriptionCopyright © 2008 Elsevier B.V. All rights reserved.
dc.description.abstractRice blast disease caused by Magnaporthe oryzae is the most important fungal disease of rice. To understand the molecular basis of interaction between the fungus and rice, we constructed a cDNA library from a rice-resistant line inoculated with M. oryzae. One hundred and fifty-three cDNA clones were sequence analyzed, of which 129 exhibited significant nucleotide sequence homology to known genes, 21 were homologous to unknown genes, while three clones did not match to any database. However, these three unmatched clones showed sequence homology at protein level in the protein databases and one of them encoded a disease resistance-related protein kinase and was abundant in the EST collection. Northern analysis showed that this disease resistance-related protein kinase gene was induced by inoculation and only expressed in the rice-resistant, but not susceptible, lines. Southern analysis showed that this gene was present in a single copy in the rice genome and co-segregated with the M. oryzae resistance in the cross of the resistant and susceptible lines. This study illustrates that sequencing of ESTs from inoculated resistant plants can reveal genes responsive to pathogen infection, which could help understand plant defense mechanisms.
dc.description.statementofresponsibilityBu-Jun Shi and Guo-Liang Wang
dc.description.urihttp://www.elsevier.com/wps/find/journaldescription.cws_home/506033/description#description
dc.identifier.citationGene, 2008; 427(1-2):80-85
dc.identifier.doi10.1016/j.gene.2008.09.015
dc.identifier.issn0378-1119
dc.identifier.issn1879-0038
dc.identifier.urihttp://hdl.handle.net/2440/53506
dc.language.isoen
dc.publisherElsevier Science BV
dc.source.urihttps://doi.org/10.1016/j.gene.2008.09.015
dc.subjectMagnaporthe
dc.subjectGenetic Predisposition to Disease
dc.subjectCalcium
dc.subjectDNA, Complementary
dc.subjectBlotting, Northern
dc.subjectBlotting, Southern
dc.subjectPlant Diseases
dc.subjectGene Expression Regulation, Fungal
dc.subjectGene Library
dc.subjectExpressed Sequence Tags
dc.subjectDatabases, Genetic
dc.subjectImmunity, Innate
dc.subjectOryza
dc.titleComparative study of genes expressed from rice fungus-resistant and susceptible lines during interactions with Magnaporthe oryzae
dc.typeJournal article
pubs.publication-statusPublished

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