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Fluorescence microscopy-an outline of hardware, biological handling, and fluorophore considerations

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dc.contributor.authorHickey, S.M.
dc.contributor.authorUng, B.
dc.contributor.authorBader, C.
dc.contributor.authorBrooks, R.
dc.contributor.authorLazniewska, J.
dc.contributor.authorJohnson, I.R.D.
dc.contributor.authorSorvina, A.
dc.contributor.authorLogan, J.
dc.contributor.authorMartini, C.
dc.contributor.authorMoore, C.R.
dc.contributor.authorKarageorgos, L.
dc.contributor.authorSweetman, M.J.
dc.contributor.authorBrooks, D.A.
dc.date.issued2022
dc.descriptionData source: Supplementary Materials, https://www.mdpi.com/article/10.3390/cells11010035/s1:
dc.description.abstractFluorescence microscopy has become a critical tool for researchers to understand biological processes at the cellular level. Micrographs from fixed and live-cell imaging procedures feature in a plethora of scientific articles for the field of cell biology, but the complexities of fluorescence microscopy as an imaging tool can sometimes be overlooked or misunderstood. This review seeks to cover the three fundamental considerations when designing fluorescence microscopy experiments: (1) hardware availability; (2) amenability of biological models to fluorescence microscopy; and (3) suitability of imaging agents for intended applications. This review will help equip the reader to make judicious decisions when designing fluorescence microscopy experiments that deliver high-resolution and informative images for cell biology.
dc.identifier.citationCells, 2022; 11(35):1-32
dc.identifier.doi10.3390/cells11010035
dc.identifier.issn2073-4409
dc.identifier.issn2073-4409
dc.identifier.orcidHickey, S.M. [0000-0002-3063-531X]
dc.identifier.orcidMoore, C.R. [0000-0003-3675-4026]
dc.identifier.orcidBrooks, D.A. [0000-0001-9098-3626]
dc.identifier.urihttps://hdl.handle.net/11541.2/28412
dc.language.isoen
dc.publisherMDPI AG
dc.relation.fundingEnvision Sciences
dc.rightsCopyright 2021 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY 4.0) license (https://creativecommons.org/licenses/by/4.0/)
dc.source.urihttp://dx.doi.org/10.3390/cells11010035
dc.subjectfluorescence microscopy
dc.subjectmicroscopy techniques
dc.subjectimaging agents
dc.subjectcellular imaging
dc.titleFluorescence microscopy-an outline of hardware, biological handling, and fluorophore considerations
dc.typeJournal article
pubs.publication-statusPublished
ror.fileinfo12243664560001831 13243664550001831 cells-11-00035
ror.mmsid9916629825601831

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