Immunoexpression of the relaxin receptor LGR7 in breast and uterine tissues of humans and primates

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dc.contributor.authorIvell, R.
dc.contributor.authorBalvers, M.
dc.contributor.authorPohnke, Y.
dc.contributor.authorTelgmann, R.
dc.contributor.authorBartsch, O.
dc.contributor.authorMilde-Langosch, K.
dc.contributor.authorBamberger, A.
dc.contributor.authorEinspanier, A.
dc.date.issued2003
dc.description© 2003 Ivell et al; licensee BioMed Central Ltd. This is an Open Access article: verbatim copying and redistribution of this article are permitted in all media for any purpose, provided this notice is preserved along with the article's original URL.
dc.description.abstractMETHODS: Three peptide sequences were identified from the proposed open reading frame of the cloned LGR7 receptor gene, representing both extracellular and intracellular domains. Two to three rabbits were immunized for each epitope, and the resulting sera subjected to a systematic validation using cultured cells transiently transfected with a receptor-expressing gene construct, or appropriate control constructs. RESULTS: Human and monkey (marmoset, macaque) endometrium showed consistent and specific immunostaining in the stromal cells close to glands. Staining appeared to be more intense in the luteal phase of the cycle. Weak immunostaining was also evident in the endometrial epithelial cells of the marmoset. A myoma in one patient exhibited strong immunostaining in the circumscribing connective tissue. Uterine expression was supported by RT-PCR results from cultured primary endometrial and myometrial cells. Human breast tissue (healthy and tumors) consistently indicated specific immunostaining in the interstitial connective (stromal) tissue within the glands, but not in epithelial or myoepithelial cells, except in some tumors, where a few epithelial and tumor cells also showed weak epitope expression. CONCLUSIONS: Using validated monotypic antibodies recognizing different epitopes of the LGR7 receptor, and from different immunized animals, and in different primate species, a consistent pattern of LGR7 expression was observed in the stromal (connective tissue) cells of the endometrium and breast, consistent also with the known physiology of the relaxin hormone.
dc.description.statementofresponsibilityRichard Ivell, Marga Balvers, Yvonne Pohnke, Ralph Telgmann, Olaf Bartsch, Karin Milde-Langosch, Ana-maria Bamberger, and Almuth Einspanier
dc.identifier.citationReproductive Biology and Endocrinology, 2003; 1(1):114-126
dc.identifier.doi10.1186/1477-7827-1-114
dc.identifier.issn1477-7827
dc.identifier.issn1477-7827
dc.identifier.urihttp://hdl.handle.net/2440/39067
dc.language.isoen
dc.publisherBiomed Central Ltd.
dc.source.urihttp://www.rbej.com/content/1/1/114
dc.subjectBreast
dc.subjectEndometrium
dc.subjectMammary Glands, Animal
dc.subjectCells, Cultured
dc.subjectStromal Cells
dc.subjectAnimals
dc.subjectRabbits
dc.subjectCallithrix
dc.subjectMacaca fascicularis
dc.subjectHumans
dc.subjectLeiomyoma
dc.subjectBreast Neoplasms
dc.subjectUterine Neoplasms
dc.subjectRelaxin
dc.subjectMembrane Proteins
dc.subjectReceptors, G-Protein-Coupled
dc.subjectReceptors, Peptide
dc.subjectRecombinant Fusion Proteins
dc.subjectDNA, Complementary
dc.subjectAntibodies, Monoclonal
dc.subjectImmunization
dc.subjectTransfection
dc.subjectAmino Acid Sequence
dc.subjectProtein Structure, Tertiary
dc.subjectOpen Reading Frames
dc.subjectMolecular Sequence Data
dc.subjectFemale
dc.titleImmunoexpression of the relaxin receptor LGR7 in breast and uterine tissues of humans and primates
dc.typeJournal article
pubs.publication-statusPublished

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