Molecular cloning and characterization of a cDNA encoding the human leucocyte vacuolar protein sorting (h1Vps45)

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dc.contributor.authorRajasekariah, P.
dc.contributor.authorEyre, H.
dc.contributor.authorStanley, K.
dc.contributor.authorWalls, R.
dc.contributor.authorSutherland, G.
dc.date.issued1999
dc.description.abstractWe have isolated a novel cDNA clone from human leucocyte cDNA library, encoding a Sec1p-like vacuolar protein sorting (hlVps45) which is believed to be implicated in vesicular transportation. Although the deduced amino acid (AA) sequence of this cDNA has revealed 97% identity to other known mammalian vacuolar protein sorting, there is an extensive variation in nucleotide sequence in comparison to that of three previously reported human (hVps45), rat (rVps45) and mouse (mVps45) vacuolar protein sorting (Vps45) cDNAs [ [1], [2] and [3]]. At the nucleotide sequence level hlVps45 demonstrated 90% homology to the hVps45 and rVps45 and 89% identity to mVps45 with no significant homology in their noncoding regions. The 2.4 Kb mRNA corresponding to the hlVps45 clone is widely distributed in a variety of human tissues expressing highest levels in peripheral blood mononuclear cells (PBMC), neutrophils, heart, spleen, and testis. The chromosomal mapping studies have demonstrated that the hlVps45 is localized to long arm of human chromosome 1 at q21–q22. Our data indicates that we have isolated, characterized and mapped a novel cDNA encoding hlVps45, which may play an important role in protein trafficking as well as have clinical significance in the release of inflammatory mediators e.g. histamine, bradykinin and cytokine release.
dc.description.statementofresponsibilityPoornima Rajasekariah, Helen J Eyre, Keith K Stanley, Ronald S Walls, Grant R Sutherland
dc.identifier.citationInternational Journal of Biochemistry and Cell Biology, 1999; 31(6):683-694
dc.identifier.doi10.1016/S1357-2725(99)00017-5
dc.identifier.issn1357-2725
dc.identifier.issn1878-5875
dc.identifier.urihttp://hdl.handle.net/2440/7412
dc.language.isoen
dc.publisherPERGAMON-ELSEVIER SCIENCE LTD
dc.source.urihttps://doi.org/10.1016/s1357-2725(99)00017-5
dc.subjectLeukocytes
dc.subjectChromosomes, Human, Pair 1
dc.subjectGolgi Apparatus
dc.subjectHumans
dc.subjectCarrier Proteins
dc.subjectMembrane Proteins
dc.subjectVesicular Transport Proteins
dc.subjectDNA, Complementary
dc.subjectRNA, Messenger
dc.subjectChromosome Mapping
dc.subjectCloning, Molecular
dc.subjectReverse Transcriptase Polymerase Chain Reaction
dc.subjectSequence Alignment
dc.subjectAmino Acid Sequence
dc.subjectBase Sequence
dc.subjectBiological Transport
dc.subjectMolecular Sequence Data
dc.titleMolecular cloning and characterization of a cDNA encoding the human leucocyte vacuolar protein sorting (h1Vps45)
dc.typeJournal article
pubs.publication-statusPublished

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