The effect of dietary calcium on 1,25(OH)2D3 synthesis and sparing of serum 25(OH)D3 levels

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2010

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Anderson, P.
Lee, A.
Anderson, S.
Sawyer, R.
O'Loughlin, P.
Morris, H.

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The Journal of Steroid Biochemistry and Molecular Biology, 2010; 121(1-2):288-292

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Paul H. Anderson, Alice M. Lee, Sarah M. Anderson, Rebecca K. Sawyer, Peter D. O’Loughlin and Howard A. Morris

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Abstract

Vitamin D depletion in rats causes osteopenia in at least three skeletal sites. However it is unclear whether modulation of dietary calcium intake impacts on the relationship between the level of serum 25-hydroxyvitamin D (25D) and bone loss. Nine-month-old female Sprague–Dawley rats (n = 5–6/group) were pair-fed a semi-synthetic diet containing either 0 or 20 IU vitamin D3/day with either low (0.1%) or high (1%) dietary Ca for 6 months. At 15 months of age, fasting bloods were collected for biochemical analyses. Serum 25D levels were lowest in the animals fed 0 IU vitamin D and 0.1% Ca. The animals fed 1% Ca had significantly higher serum 25D levels when compared to animals fed 0.1% Ca (P < 0.05). The major determinants of serum 25D were dietary vitamin D and dietary calcium (Multiple R = 0.75, P < 0.05). Animals fed 0.1% Ca had higher renal CYP27B1 mRNA expression and 12–18-fold increased levels of serum 1,25D. Hence, the reported effects of low calcium diets on bone loss may be, in part, due to the subsequent effects of 25D metabolism leading to reduction in vitamin D status. Such an interaction has significant implications, given the recent evidence for local synthesis of active vitamin D in bone tissue.

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Copyright © 2010 Elsevier Ltd All rights reserved.

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