Resistance of microbial populations in DDT-contaminated and uncontaminated soils

Date

2001

Authors

Kantachote, D.
Naidu, Ravendra
Singleton, Ian
McClure, Nicholas Charles
Harch, Bronwyn D.

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Journal article

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Applied Soil Ecology, 2001; 16(1):85-90

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D Kantachote, R Naidu, I Singleton, N McClure and B.D Harch

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Abstract

One DDT-contaminated soil and two uncontaminated soils were used to enumerate DDT-resistant microbes (bacteria, actinomycetes and fungi) by using soil dilution agar plates in media either with 150 μg DDT ml−1 or without DDT at different temperatures (25, 37 and 55°C). Microbial populations in this study were significantly (p<0.001) affected by DDT in the growth medium. However, the numbers of microbes in long-term contaminated and uncontaminated soils were similar, presumably indicating that DDT-resistant microbes had developed over a long time exposure. The tolerance of isolated soil microbes to DDT varied in the order fungi>actinomycetes>bacteria. Bacteria from contaminated soil were more resistant to DDT than bacteria from uncontaminated soils. Microbes isolated at different temperatures also demonstrated varying degrees of DDT resistance. For example, bacteria and actinomycetes isolated at all incubation temperatures were sensitive to DDT. Conversely fungi isolated at all temperatures were unaffected by DDT.

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School of Earth and Environmental Sciences : Soil and Land Systems

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Copyright © 2001 Elsevier Science B.V. All rights reserved.

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