Mixed subunit capsids can be assembled in vitro with coat protein subunits from two cucumoviruses
Date
1995
Authors
Chen, Bao-Dong
Randles, John Wesley
Francki, R. I. B.
Editors
Advisors
Journal Title
Journal ISSN
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Type:
Journal article
Citation
Journal of General Virology, 1995; 76(4):971-973
Statement of Responsibility
B. Chen, J. W. Randles and R. I. B. Francki
Conference Name
Abstract
Virus particles were reassembled in vitro from tomato aspermy virus strain V (V-TAV) RNA and a mixture of subunits prepared from V-TAV and 35S-labelled cucumber mosaic virus strain T (T-CMV). Immunodiffusion tests showed that the reassembled particles reacted with polyclonal antisera raised against both V-TAV and T-CMV. Radioactivity was found in the precipitin line formed between the reassembled particles and antiserum raised against T-CMV as well as in the precipitin line formed between the reassembled particles and antiserum raised against V-TAV. This shows that 35S-labelled T-CMV protein subunits were incorporated with V-TAV protein subunits into the same particles. Thus, coat proteins of V-TAV and T-CMV can coassemble and form mixed-subunit capsids in vitro.
School/Discipline
School of Agriculture, Food and Wine : Plant and Food Science
School of Agriculture, Food and Wine
School of Agriculture, Food and Wine
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© 1995 SGM