Cellulose synthase-like CslF genes mediate the synthesis of cell wall (1,3;1,4)-beta-D-glucans

Simple item page
dc.contributor.authorBurton, R.
dc.contributor.authorWilson, S.
dc.contributor.authorHrmova, M.
dc.contributor.authorHarvey, A.
dc.contributor.authorShirley, N.
dc.contributor.authorMedhurst, A.
dc.contributor.authorStone, B.
dc.contributor.authorNewbigin, E.
dc.contributor.authorBacic, A.
dc.contributor.authorFincher, G.
dc.date.issued2006
dc.description.abstractA characteristic feature of grasses and commercially important cereals is the presence of (1,3;1,4)-ß-D-glucans in their cell walls. We have used comparative genomics to link a major quantitative trait locus for (1,3;1,4)-ß-D-glucan content in barley grain to a cluster of cellulose synthase–like CslF genes in rice. After insertion of rice CslF genes into Arabidopsis, we detected (1,3;1,4)-ß-D-glucan in walls of transgenic plants using specific monoclonal antibodies and enzymatic analysis. Because wild-type Arabidopsis does not contain CslF genes or have (1,3;1,4)-ß-D-glucans in its walls, these experiments provide direct, gain-of-function evidence for the participation of rice CslF genes in (1,3;1,4)-ß-D-glucan biosynthesis.
dc.description.statementofresponsibilityRachel A. Burton, Sarah M. Wilson, Maria Hrmova, Andrew J. Harvey, Neil J. Shirley, Anne Medhurst, Bruce A. Stone, Edward J. Newbigin, Antony Bacic, Geoffrey B. Fincher
dc.identifier.citationScience, 2006; 311(5769):1940-1942
dc.identifier.doi10.1126/science.1122975
dc.identifier.issn0036-8075
dc.identifier.issn1095-9203
dc.identifier.orcidBurton, R. [0000-0002-0638-4709]
dc.identifier.orcidHrmova, M. [0000-0002-3545-0605]
dc.identifier.orcidShirley, N. [0000-0001-8114-9891]
dc.identifier.urihttp://hdl.handle.net/2440/23834
dc.language.isoen
dc.publisherAmer Assoc Advancement Science
dc.source.urihttps://doi.org/10.1126/science.1122975
dc.subjectCell Wall
dc.subjectPlants, Genetically Modified
dc.subjectArabidopsis
dc.subjectHordeum
dc.subjectPlant Leaves
dc.subjectGlucosyltransferases
dc.subjectbeta-Glucans
dc.subjectAntibodies, Monoclonal
dc.subjectGenomics
dc.subjectTransformation, Genetic
dc.subjectSynteny
dc.subjectGenes, Plant
dc.subjectGenome, Plant
dc.subjectQuantitative Trait Loci
dc.subjectOryza
dc.titleCellulose synthase-like CslF genes mediate the synthesis of cell wall (1,3;1,4)-beta-D-glucans
dc.typeJournal article
pubs.publication-statusPublished

Files

Collections

Agriculture, Food and Wine publications