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The Golgi apparatus in the endomembrane-rich gastric parietal cells exist as functional stable mini-stacks dispersed throughout the cytoplasm

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dc.contributor.authorGunn, P.A.
dc.contributor.authorGliddon, B.L.
dc.contributor.authorLondrigan, S.L.
dc.contributor.authorLew, A.M.
dc.contributor.authorvan Driel, I.R.
dc.contributor.authorGleeson, P.A.
dc.date.issued2011
dc.description.abstractBackground information: Acid-secreting gastric parietal cells are polarized epithelial cells that harbour highly abundant and specialized, H+,K+ ATPase-containing, tubulovesicular membranes in the apical cytoplasm. The Golgi apparatus has been implicated in the biogenesis of the tubulovesicular membranes; however, an unanswered question is how a typical Golgi organization could regulate normal membrane transport within the membrane-dense cytoplasm of parietal cells. Results: Here, we demonstrate that the Golgi apparatus of parietal cells is not the typical juxta-nuclear ribbon of stacks, but rather individual Golgi units are scattered throughout the cytoplasm. The Golgi membrane structures labelled with markers of both cis- and trans-Golgi membrane, indicating the presence of intact Golgi stacks. The parietal cell Golgi stacks were closely aligned with the microtubule network and were shown to participate in both anterograde and retrograde transport pathways. Dispersed Golgi stacks were also observed in parietal cells from H+,K+ ATPase-deficient mice that lack tubulovesicular membranes. Conclusions: These results indicate that the unusual organization of individual Golgi stacks dispersed throughout the cytoplasm of these terminally differentiated cells is likely to be a developmentally regulated event.
dc.identifier.citationBiology of the Cell, 2011; 103(12):559-572
dc.identifier.doi10.1042/BC20110074
dc.identifier.issn0248-4900
dc.identifier.issn1768-322X
dc.identifier.urihttps://hdl.handle.net/1959.8/155456
dc.language.isoen
dc.publisherJohn Wiley & Sons
dc.relation.fundingARC
dc.relation.fundingNHMRC
dc.relation.fundingJuvenile Diabetes Research Foundation
dc.rightsCopyright 2011 The Author(s). The author(s) has paid for this article to be freely available under the terms of the Creative Commons Attribution Non-Commercial Licence (http://creativecommons.org/licenses/by-nc/2.5/) which permits unrestricted non-commercial use, distribution and reproduction in any medium, provided the original work is properly cited. (http://creativecommons.org/licenses/by-nc/2.5/)
dc.source.urihttps://doi.org/10.1042/BC20110074
dc.subjectacid secretion
dc.subjectgolgi apparatus
dc.subjectgolgi fragmentation
dc.subjectparietal cell
dc.subjectretrograde transport
dc.titleThe Golgi apparatus in the endomembrane-rich gastric parietal cells exist as functional stable mini-stacks dispersed throughout the cytoplasm
dc.typeJournal article
pubs.publication-statusPublished
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ror.mmsid9915909142101831

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