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Development of advanced dressings for the delivery of progenitor cells

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dc.contributor.authorKirby, G.T.S.
dc.contributor.authorMills, S.J.
dc.contributor.authorVandenpoel, L.
dc.contributor.authorPinxteren, J.
dc.contributor.authorTing, A.
dc.contributor.authorShort, R.D.
dc.contributor.authorCowin, A.J.
dc.contributor.authorMichelmore, A.
dc.contributor.authorSmith, L.E.
dc.date.issued2017
dc.descriptionData source: Supporting information, https://doi.org/10.1021/acsami.6b14725
dc.description.abstractCulture surfaces that substantially reduce the degree of cell manipulation in the delivery of cell sheets to patients are described. These surfaces support the attachment, culture, and delivery of multipotent adult progenitor cells (MAPC). It was essential that the processes of attachment/detachment to the surface did not affect cell phenotype nor the function of the cultured cells. Both acid-based and aminebased surface coatings were generated from acrylic acid, propanoic acid, diaminopropane, and heptylamine precursors, respectively. While both functional groups supported cell attachment/detachment, amine coated surfaces gave optimal performance. X-ray photoelectron spectroscopy (XPS) showed that at a primary amine to carbon surface ratio of between 0.01 and 0.02, greater than 90% of attached cells were effectively transferred to a model wound bed. A dependence on primary amine concentration has not previously been reported. After 48 h of culture on the optimized amine surface, PCR, functional, and viability assays showed that MAPC retained their stem cell phenotype, full metabolic activity, and biological function. Consequently, in a proof of concept experiment, it was shown that this amine surface when coated onto a surgical dressing provides an effective and simple technology for the delivery of MAPC to murine dorsal excisional wounds, with MAPC delivery verified histologically. By optimizing for cell delivery using a combination of in vitro and in vivo techniques, we developed an effective surface for the delivery of MAPC in a clinically relevant format.
dc.identifier.citationACS applied materials & interfaces, 2017; 9(4):3445-3454
dc.identifier.doi10.1021/acsami.6b14725
dc.identifier.issn1944-8244
dc.identifier.issn1944-8252
dc.identifier.orcidCowin, A.J. [0000-0003-2885-2080]
dc.identifier.urihttps://hdl.handle.net/11541.2/124759
dc.language.isoen
dc.publisherAmerican Chemical Society
dc.rightsCopyright 2017 American Chemical Society
dc.source.urihttps://doi.org/10.1021/acsami.6b14725
dc.subjectcell delivery
dc.subjectcell therapy
dc.subjectchronic wounds
dc.subjectprogenitor cell
dc.subjectsurface modification
dc.subjectCells, Cultured
dc.subjectStem Cells
dc.subjectMultipotent Stem Cells
dc.subjectAnimals
dc.subjectHumans
dc.subjectMice
dc.subjectBandages
dc.subjectAdult Stem Cells
dc.titleDevelopment of advanced dressings for the delivery of progenitor cells
dc.typeJournal article
pubs.publication-statusPublished
ror.mmsid9916113311901831

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