Increased oral virulence of Escherichia coli expressing a variant Shiga-like toxin type II operon is associated with both A subunit residues Met4 and Gly102

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dc.contributor.authorPaton, A.
dc.contributor.authorManning, P.
dc.contributor.authorPaton, J.
dc.date.issued1995
dc.description.abstractWe have previously demonstrated that Escherichia coli DH5 alpha clones expressing closely-related Shiga-like toxin type II operons (designated SLT-II/OX3b and SLT-II/O48) had similar cytotoxicity for Vero cells, but differed in oral virulence for streptomycin-treated mice. Studies with chimeric toxin operons indicated that increased virulence was associated with the A subunit of SLT-II/OX3b, which differs from that of SLT-II/O48 by two amino acids (at positions 4 and 102). In the present study, we have constructed a series of additional chimeric derivatives of the SLT-II/OX3b and SLT-II/O48 operons and assessed the effect of single A subunit amino acid substitutions on oral virulence. Maximal virulence, as judged by median survival time after oral challenge, was associated only with the combination of Met4 and Gly102, as found in the A subunit of SLT-II/OX3b.
dc.identifier.citationMicrobial Pathogenesis, 1995; 19(3):185-191
dc.identifier.doi10.1006/mpat.1995.0057
dc.identifier.issn0882-4010
dc.identifier.issn1096-1208
dc.identifier.orcidPaton, J. [0000-0001-9807-5278]
dc.identifier.urihttp://hdl.handle.net/2440/11641
dc.language.isoen
dc.publisherAcademic Press Limited
dc.source.urihttps://doi.org/10.1006/mpat.1995.0057
dc.subjectAnimals
dc.subjectMice, Inbred BALB C
dc.subjectMice
dc.subjectEscherichia coli
dc.subjectMethionine
dc.subjectGlycine
dc.subjectBacterial Toxins
dc.subjectShiga Toxins
dc.subjectAdministration, Oral
dc.subjectVirulence
dc.subjectStructure-Activity Relationship
dc.subjectOperon
dc.subjectMale
dc.titleIncreased oral virulence of Escherichia coli expressing a variant Shiga-like toxin type II operon is associated with both A subunit residues Met4 and Gly102
dc.typeJournal article
pubs.publication-statusPublished

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