A Lycopersicon esculentum phosphate transporter (LePT1) involved in phosphorus uptake from a vesicular-arbuscular mycorrhizal fungus

Simple item page
dc.contributor.authorRosewarne, G.
dc.contributor.authorBarker, S.
dc.contributor.authorSmith, S.
dc.contributor.authorSmith, F.
dc.contributor.authorSchachtman, D.
dc.date.issued1999
dc.description.abstractIn vesicular–arbuscular mycorrhizal symbioses, specialized fungal structures (the arbuscules) are formed which are in intimate contact with plant root cortical cells. It is assumed that these arbuscules are the major sites of solute transfer between the plant and fungus, but there have been no studies that definitively show the extent or types of transfer processes that occur in this structure. Phosphate is one of the major nutrients that is acquired by mycorrhizal fungi and transferred to plants. In this study a single Lycopersicon esculentum cDNA was cloned and shown to be identical to LePT1, a previously cloned inorganic-phosphate transporter. Expression studies revealed that LePT1 transcript levels remained constant in mycorrhizal plants, but increased in phosphate-starved, non-mycorrhizal plants. Localization of the LePT1 transcript by in situ hybridization showed that this gene is highly expressed in arbuscule-containing cortical cells in mycorrhizal plants. In non-mycorrhizal plants LePT1 expression was localized to the stele and cortex. The expression studies suggest that this transporter is involved in phosphate nutrition of L. esculentum and its localization in cells that contain arbuscules indicate that it may be the mechanism used by the plant to take up phosphate that is effluxed across the fungal plasma membrane of the arbuscule. Based on our findings and those of others, an integrated model of inorganic phosphate uptake and transfer in mycorrhizal and non-mycorrhizal plants is presented.
dc.description.statementofresponsibilityGarry M. Rosewarne, Susan J. Barker, Sally E. Smith, F. Andrew Smith & Daniel P. Schachtman
dc.identifier.citationNew Phytologist, 1999; 144(3):507-516
dc.identifier.doi10.1046/j.1469-8137.1999.00536.x
dc.identifier.issn0028-646X
dc.identifier.issn1469-8137
dc.identifier.urihttp://hdl.handle.net/2440/13476
dc.language.isoen
dc.provenancePublished Online: 4 Jan 2002
dc.publisherBlackwell / Wiley
dc.rightsCopyright status unknown
dc.source.urihttps://doi.org/10.1046/j.1469-8137.1999.00536.x
dc.subjectmycorrhiza; transport; phosphate; tomato; in situ RNA localization; arbuscule
dc.titleA Lycopersicon esculentum phosphate transporter (LePT1) involved in phosphorus uptake from a vesicular-arbuscular mycorrhizal fungus
dc.typeJournal article
pubs.publication-statusPublished

Files

Collections

Agriculture, Food and Wine publications