Preparation of water soluble L-arginine capped CdSe/ZnS QDs and their interaction with synthetic DNA: picosecond-resolved FRET study
Date
2012
Authors
Giri, A.
Goswami, N.
Lemmens, P.
Pal, S.K.
Editors
Advisors
Journal Title
Journal ISSN
Volume Title
Type:
Journal article
Citation
Materials Research Bulletin, 2012; 47(8):1912-1918
Statement of Responsibility
Conference Name
Abstract
We have exchanged TOPO (trioctylphosphine oxide) ligand of CdSe/ZnS core/shell quantum dots (QDs) with an amino acid L-arginine (Arg) at the toluene/water interface and eventually rendered the QDs from toluene to aqueous phase. We have studied the interaction of the water soluble Arg-capped QDs (energy donor) with ethidium (EB) labeled synthetic dodecamer DNA (energy acceptor) using picoseconds resolved Forster resonance energy transfer (FRET) technique.
Furthermore, we have applied a model developed by M. Tachiya to understand the kinetics of energy transfer and the distribution of acceptor (EB-DNA) molecules around the donor QDs. Circular dichroism (CD) studies revealed a negligible perturbation in the native B-form structure of the DNA upon interaction with Arg-capped QDs. The melting and the rehybridization pathways of the DNA attached to the QDs have been monitored by the CD which reveals hydrogen bonding is the associative mechanism for interaction between Arg-capped QDs and DNA.
School/Discipline
Dissertation Note
Provenance
Description
Data source: Figures & tables, https://doi-org/10.1016/j.materresbull.2012.04.025
Access Status
Rights
Copyright 2012 Elsevier