Estimation of the volume of blood in a small disc punched from a dried blood spot card

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dc.contributor.authorHewawasam, E.
dc.contributor.authorLiu, G.
dc.contributor.authorJeffery, D.
dc.contributor.authorGibson,, R.
dc.contributor.authorMuhlhausler, B.
dc.date.issued2018
dc.description.abstractA specialized dried blood spot (DBS) collection system (PUFAcoat™) in combination with liquid chromatography-tandem mass spectrometry (LC-MS/ MS) has enabled the measurement of numerous analytes in minimal volumes of blood. The current study aimed to determine the volume of blood in 3 and 6mm discs obtained from our DBS system. The volume of blood in 3 and 6mm discs obtained from DBS cards is estimated using four different methods: (i) gravimetric analysis; (ii) LC-MS/MS; (iii) a hemoglobin colorimetric assay; and (iv) GC. Differences in the estimated volume are compared between methods, and variations in estimated blood volume within and between individuals are determined. The average volume of blood in a DBS disc is calculated to be 1.6±0.4 μL and 8.7±1.9 μL for 3 and 6mm discs, respectively. This estimate is similar between direct and indirect analytical methods and between DBS samples with different starting volumes independent of the method, but there is considerable variation in the volume of blood in comparably-sized DBS discs from different individuals. Current methods enable the estimation of the blood volume in a small disc obtained from a DBS but a method that can both accurately measure volume and store blood on a DBS is required. Practical Applications: This study demonstrates that it is possible to evaluate the volume of blood contained in a small disc punched from a dried blood spot (DBS) card using a range of different methods, but there is considerable variation in the estimate of volume in samples collected from different individuals. These variations could potentially result in overestimation or underestimation of endogenous levels of various metabolites if presuming a fixed volume, which may be clinically significant. Although there are several ways to correct for blood volume contained on a DBS, the practicality and the universality of these methods are questionable. Ideally, the development of a tool to better determine the volume of blood and/or to precisely spot a volume of blood is required to ensure accuracy when expressing the results of DBS analyses per unit of blood.
dc.description.statementofresponsibilityErandi Hewawasam, Ge Liu, David W. Jeffery, Robert A. Gibson and Beverly S. Muhlhausler
dc.identifier.citationEuropean Journal of Lipid Science and Technology, 2018; 120(3):1700362-1-1700362-6
dc.identifier.doi10.1002/ejlt.201700362
dc.identifier.issn1438-7697
dc.identifier.issn1438-9312
dc.identifier.orcidHewawasam, E. [0000-0002-8320-3668]
dc.identifier.orcidJeffery, D. [0000-0002-7054-0374]
dc.identifier.orcidMuhlhausler, B. [0000-0002-9021-6790]
dc.identifier.urihttp://hdl.handle.net/2440/111651
dc.language.isoen
dc.publisherWiley
dc.relation.granthttp://purl.org/au-research/grants/nhmrc/1083009
dc.relation.granthttp://purl.org/au-research/grants/nhmrc/1046207
dc.relation.granthttp://purl.org/au-research/grants/nhmrc/1035530
dc.rights© 2017 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim
dc.source.urihttps://doi.org/10.1002/ejlt.201700362
dc.subjectPUFAcoat paper; volume estimation; dried blood spot; haematocrit; comparison of methods
dc.titleEstimation of the volume of blood in a small disc punched from a dried blood spot card
dc.typeJournal article
pubs.publication-statusPublished

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