Single-cell immune profiling in coronary artery disease: the role of state-of-the-art immunophenotyping with mass cytometry in the diagnosis of atherosclerosis

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dc.contributor.authorKott, K.A.
dc.contributor.authorVernon, S.T.
dc.contributor.authorHansen, T.
dc.contributor.authorde Dreu, M.
dc.contributor.authorDas, S.K.
dc.contributor.authorPowell, J.
dc.contributor.authorFazekas de St Groth, B.
dc.contributor.authorDi Bartolo, B.A.
dc.contributor.authorMcGuire, H.M.
dc.contributor.authorFigtree, G.A.
dc.date.issued2020
dc.description.abstractCoronary artery disease remains the leading cause of death globally and is a major burden to every health system in the world. There have been significant improvements in risk modification, treatments, and mortality; however, our ability to detect asymptomatic disease for early intervention remains limited. Recent discoveries regarding the inflammatory nature of atherosclerosis have prompted investigation into new methods of diagnosis and treatment of coronary artery disease. This article reviews some of the highlights of the important developments in cardioimmunology and summarizes the clinical evidence linking the immune system and atherosclerosis. It provides an overview of the major serological biomarkers that have been associated with atherosclerosis, noting the limitations of these markers attributable to low specificity, and then contrasts these serological markers with the circulating immune cell subtypes that have been found to be altered in coronary artery disease. This review then outlines the technique of mass cytometry and its ability to provide high‐dimensional single‐cell data and explores how this high‐resolution quantification of specific immune cell subpopulations may assist in the diagnosis of early atherosclerosis in combination with other complimentary techniques such as single‐cell RNA sequencing. We propose that this improved specificity has the potential to transform the detection of coronary artery disease in its early phases, facilitating targeted preventative approaches in the precision medicine era.
dc.description.statementofresponsibilityKatharine A. Kott, Stephen T. Vernon, Thomas Hansen, Macha de Dreu, Souvik K. Das, Joseph Powell, Barbara Fazekas de St Groth, Belinda A. Di Bartolo, Helen M. McGuire, Gemma A. Figtree
dc.identifier.citationJournal of the American Heart Association, 2020; 9(24):e017759-1-e017759-16
dc.identifier.doi10.1161/JAHA.120.017759
dc.identifier.issn2047-9980
dc.identifier.issn2047-9980
dc.identifier.orcidDi Bartolo, B.A. [0000-0001-8420-0461]
dc.identifier.urihttp://hdl.handle.net/2440/130507
dc.language.isoen
dc.publisherWiley
dc.relation.granthttp://purl.org/au-research/grants/nhmrc/1175781
dc.relation.granthttp://purl.org/au-research/grants/nhmrc/11359290
dc.rights© 2020 The Authors. Published on behalf of the American Heart Association, Inc., by Wiley Blackwell This is an open access article under the terms of the Creative Commons Attribution‐NonCommercial‐NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non‐commercial and no modifications or adaptations are made.
dc.source.urihttps://doi.org/10.1161/jaha.120.017759
dc.subjectAtherosclerosis; coronary artery disease; immune system; inflammation; mass cytometry
dc.titleSingle-cell immune profiling in coronary artery disease: the role of state-of-the-art immunophenotyping with mass cytometry in the diagnosis of atherosclerosis
dc.typeJournal article
pubs.publication-statusPublished

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