Changes in bacterial denitrifier community abundance over time in an agricultural field and their relationship with denitrification activity
Date
2008
Authors
Dandie, C.E.
Burton, D.L.
Zebarth, B.J.
Henderson, S.L.
Trevors, J.T.
Goyer, C.
Editors
Advisors
Journal Title
Journal ISSN
Volume Title
Type:
Journal article
Citation
Applied and Environmental Microbiology, 2008; 74(19):5997-6005
Statement of Responsibility
Conference Name
Abstract
<jats:title>ABSTRACT</jats:title>
<jats:p>
This study measured total bacterial and denitrifier community abundances over time in an agricultural soil cropped to potatoes (
<jats:italic>Solanum tuberosum</jats:italic>
L.) by using quantitative PCR. Samples were collected on 10 dates from spring to autumn and from three spatial locations: in the potato “hill” between plants (H), close to the plant (H
<jats:sub>p</jats:sub>
), and in the “furrow” (F). The denitrification rates, N
<jats:sub>2</jats:sub>
O emissions, and environmental parameters were also measured. Changes in denitrifier abundance over time and spatial location were small (1.7- to 2.7-fold for the
<jats:italic>nirK</jats:italic>
,
<jats:italic>nosZ</jats:italic>
, and
<jats:italic>cnorB</jats:italic>
<jats:sub>B</jats:sub>
guilds), whereas the
<jats:italic>cnorB</jats:italic>
<jats:sub>P</jats:sub>
community (
<jats:italic>Pseudomonas mandelii</jats:italic>
and closely related spp.) showed an ∼4.6-fold change. The seasonal patterns of denitrifier gene numbers varied with the specific community: lower
<jats:italic>nosZ</jats:italic>
gene numbers in April and May than in June and July, higher
<jats:italic>cnorB</jats:italic>
<jats:sub>P</jats:sub>
gene numbers in May and June than in March and April and September and November, higher
<jats:italic>nirK</jats:italic>
gene numbers in early spring than in late autumn, and no change in
<jats:italic>cnorB</jats:italic>
<jats:sub>B</jats:sub>
gene numbers. Gene numbers were higher for the H
<jats:sub>p</jats:sub>
than the H location for the
<jats:italic>nosZ</jats:italic>
and
<jats:italic>nirK</jats:italic>
communities and for the
<jats:italic>cnorB</jats:italic>
<jats:sub>P</jats:sub>
community on individual dates, presumably indicating an effect of the plant on denitrifier abundance. Higher
<jats:italic>cnorB</jats:italic>
<jats:sub>P</jats:sub>
gene numbers for the H location than the F location and for
<jats:italic>nosZ</jats:italic>
and
<jats:italic>cnorB</jats:italic>
<jats:sub>B</jats:sub>
on individual dates reflect the effect of spatial location on abundance. Denitrifier abundance changes were not related to any environmental parameter, although a weak relationship exists between
<jats:italic>cnorB</jats:italic>
<jats:sub>P</jats:sub>
gene numbers, extractable organic carbon values, and temperature. Denitrification and N
<jats:sub>2</jats:sub>
O emissions were mostly regulated by inorganic nitrogen availability and water-filled pore space but were uncoupled from denitrifier community abundances measured in this system.
</jats:p>
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Link to a related website: http://europepmc.org/articles/pmc2565952?pdf=render, Open Access via Unpaywall
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Copyright 2008 American Society for Microbiology