SOX3 promotes generation of committed spermatogonia in postnatal mouse testes

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dc.contributor.authorMcAninch, D.
dc.contributor.authorMäkelä, J.-A.
dc.contributor.authorLa, H.M.
dc.contributor.authorHughes, J.N.
dc.contributor.authorLovell-Badge, R.
dc.contributor.authorHobbs, R.M.
dc.contributor.authorThomas, P.Q.
dc.date.issued2020
dc.description.abstractSOX3 is a transcription factor expressed within the developing and adult nervous system where it mostly functions to help maintain neural precursors. Sox3 is also expressed in other locations, notably within the spermatogonial stem/progenitor cell population in postnatal testis. Independent studies have shown that Sox3 null mice exhibit a spermatogenic block as young adults, the mechanism of which remains poorly understood. Using a panel of spermatogonial cell marker genes, we demonstrate that Sox3 is expressed within the committed progenitor fraction of the undifferentiated spermatogonial pool. Additionally, we use a Sox3 null mouse model to define a potential role for this factor in progenitor cell function. We demonstrate that Sox3 expression is required for transition of undifferentiated cells from a GFRα1+ self-renewing state to the NGN3 + transit-amplifying compartment. Critically, using chromatin immunoprecipitation, we demonstrate that SOX3 binds to a highly conserved region in the Ngn3 promoter region in vivo, indicating that Ngn3 is a direct target of SOX3. Together these studies indicate that SOX3 functions as a pro-commitment factor in spermatogonial stem/progenitor cells.
dc.description.statementofresponsibilityDale McAninch, Juho-Antti Mäkelä, Hue M. La, James N. Hughes, Robin Lovell-Badge, Robin M. Hobbs, Paul Q. Thomas
dc.identifier.citationScientific Reports, 2020; 10(1):6751-1-6751-13
dc.identifier.doi10.1038/s41598-020-63290-3
dc.identifier.issn2045-2322
dc.identifier.issn2045-2322
dc.identifier.orcidMcAninch, D. [0000-0001-9735-6553]
dc.identifier.orcidHobbs, R.M. [0000-0002-3853-2614]
dc.identifier.urihttp://hdl.handle.net/2440/124627
dc.language.isoen
dc.publisherSpringer Nature
dc.relation.granthttp://purl.org/au-research/grants/arc/#DP140103965
dc.rights© The Author(s) 2020. Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
dc.source.urihttps://doi.org/10.1038/s41598-020-63290-3
dc.subjectTestis
dc.subjectSpermatogonia
dc.subjectAnimals
dc.subjectMice, Knockout
dc.subjectMice
dc.subjectDNA-Binding Proteins
dc.subjectNerve Tissue Proteins
dc.subjectTranscription Factors
dc.subjectSignal Transduction
dc.subjectCell Differentiation
dc.subjectSpermatogenesis
dc.subjectGene Expression Regulation, Developmental
dc.subjectProtein Binding
dc.subjectMale
dc.subjectGlial Cell Line-Derived Neurotrophic Factor Receptors
dc.subjectBasic Helix-Loop-Helix Transcription Factors
dc.subjectPromoter Regions, Genetic
dc.subjectSOXB1 Transcription Factors
dc.subjectAdult Germline Stem Cells
dc.subjectPromyelocytic Leukemia Zinc Finger Protein
dc.titleSOX3 promotes generation of committed spermatogonia in postnatal mouse testes
dc.typeJournal article
pubs.publication-statusPublished

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