Advancing of 3D-Printed Titanium Implants with Combined Antibacterial Protection Using Ultrasharp Nanostructured Surface and Gallium-Releasing Agents

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dc.contributor.authorMaher, S.
dc.contributor.authorLinklater, D.
dc.contributor.authorRastin, H.
dc.contributor.authorLiao, S.T.-Y.
dc.contributor.authorMartins de Sousa, K.
dc.contributor.authorLima-Marques, L.
dc.contributor.authorKingshott, P.
dc.contributor.authorThissen, H.
dc.contributor.authorIvanova, E.P.
dc.contributor.authorLosic, D.
dc.date.issued2022
dc.description.abstractThis paper presents the development of advanced Ti implants with enhanced antibacterial activity. The implants were engineered using additive manufacturing three-dimensional (3D) printing technology followed by surface modification with electrochemical anodization and hydrothermal etching, to create unique hierarchical micro/nanosurface topographies of microspheres covered with sharp nanopillars that can mechanically kill bacteria in contact with the surface. To achieve enhanced antibacterial performance, fabricated Ti implant models were loaded with gallium nitrate as an antibacterial agent. The antibacterial efficacy of the fabricated substrates with the combined action of sharp nanopillars and locally releasing gallium ions (Ga3+) was evaluated toward Staphylococcus aureus and Pseudomonas aeruginosa. Results confirm the significant antibacterial performance of Ga3+-loaded substrates with a 100% eradication of bacteria. The nanopillars significantly reduced bacterial attachment and prevented biofilm formation while also killing any bacteria remaining on the surface. Furthermore, 3Dprinted surfaces with microspheres of diameter 5−30 μm and interspaces of 12−35 μm favored the attachment of osteoblast-like MG-63 cells, as confirmed via the assessment of their attachment, proliferation, and viability. This study provides important progress toward engineering of next-generation 3D-printed implants, that combine surface chemistry and structure to achieve a highly efficacious antibacterial surface with dual cytocompatibility to overcome the limitations of conventional Ti implants.
dc.description.statementofresponsibilityShaheer Maher, Denver Linklater, Hadi Rastin, Sandy Tzu-Ying Liao, Karolinne Martins de Sousa, Luis Lima-Marques, Peter Kingshott, Helmut Thissen, Elena P. Ivanova, and Dusan Losic
dc.identifier.citationACS Biomaterials Science & Engineering, 2022; 8(1):314-327
dc.identifier.doi10.1021/acsbiomaterials.1c01030
dc.identifier.issn2373-9878
dc.identifier.issn2373-9878
dc.identifier.orcidMaher, S. [0000-0002-2556-1096]
dc.identifier.orcidLosic, D. [0000-0002-1930-072X]
dc.identifier.urihttps://hdl.handle.net/2440/133907
dc.language.isoen
dc.publisherAmerican Chemical Society
dc.relation.granthttp://purl.org/au-research/grants/arc/IH15000003
dc.relation.granthttp://purl.org/au-research/grants/arc/IH130100017
dc.relation.granthttp://purl.org/au-research/grants/arc/IC180100005
dc.rights© 2021, American Chemical Society
dc.source.urihttps://doi.org/10.1021/acsbiomaterials.1c01030
dc.subjecttitanium implants
dc.subjectantibacterial surfaces
dc.subjectosseointegration
dc.subjectadditive manufacturing
dc.subjectselective laser melting
dc.subjectelectrochemical anodization
dc.subjecthydrothermal
dc.subjectgallium nitrate
dc.titleAdvancing of 3D-Printed Titanium Implants with Combined Antibacterial Protection Using Ultrasharp Nanostructured Surface and Gallium-Releasing Agents
dc.typeJournal article
pubs.publication-statusPublished

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